Insights into the Mechanistic Role of the [Fe4S4] Cubane in the A-Cluster {[Fe4S4]-(SR)-[NipNid]} of Acetyl-Coenzyme A Synthase

被引：7

作者：

Liu, Yi ^{[1
,2
]}

Wang, Feng ^{[3
]}

Li, Pingwei ^{[4
]}

Tan, Xiangshi ^{[1
,2
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

[3] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[4] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA

来源：

CHEMBIOCHEM | 2011年 / 12卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Fe4S4] cubane; NipNid] site; acetyl-coenzyme A synthase; crystal structure; metalloenzymes; CARBON-MONOXIDE DEHYDROGENASE; COA SYNTHASE; ALPHA-SUBUNIT; CLOSTRIDIUM-THERMOACETICUM; ELECTRONIC-PROPERTIES; METHYL TRANSFER; PROTEIN; NICKEL; MODEL; MOSSBAUER;

D O I：

10.1002/cbic.201100101

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cubane revolution: We have constructed various mutants to probe the role of [Fe4S4] cubane. The results show that [Fe4S4] cubane, which is bridged to a [NipNid] center via Cys509 cysteinate in the A-cluster of acetyl-coenzyme A synthase (see figure), modulates the redox properties of the catalytic metal Nip and stabilizes the low valence of Nip through the {[Fe4S4]-(SR)-Nip} "conjugation system". © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1417 / 1421

页数：5

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