Keratin 14 and 19 expression in normal, dysplastic and malignant oral epithelia. A study using in situ hybridization and immunohistochemistry

被引:54
作者
Su, L
Morgan, PR
Lane, EB
机构
[1] GUYS HOSP,UMDS,DEPT ORAL MED & PATHOL,LONDON SE1,ENGLAND
[2] EMORY UNIV,SCH MED,DIV ORAL HEAD & NECK PATHOL,DECATUR,GA
[3] UNIV DUNDEE,INST MED SCI,DEPT PHYSIOL & ANAT,DUNDEE DD1 4HN,SCOTLAND
关键词
carcinoma; in situ hybridization; keratins; mRNA; mucosal epithelium; riboprobes;
D O I
10.1111/j.1600-0714.1996.tb00265.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Specific mRNA and protein for two major keratins, K14 and K19, were investigated in normal, dysplastic and malignant oral epithelia by combined iii situ hybridization and immunohistochemistry. In normal epithelia, K14 mRNA and protein were present almost exclusively in the basal layer of non-cornified, and in rete-processes of comified, sites. Dysplastic epithelium showed irregular extension of the K14 transcript and protein into superficial cells. In squamous cell carcinoma (SCC), K14 transcript was abundant in most samples whilst in one poorly differentiated carcinoma mRNA but no protein was detected, K19 mRNA and its protein were present predominantly in basal cells of noncomified epithelium, whereas in cornified epithelium only mRNA was detected. In dysplasias, K19 transcript was detected in all specimens but its protein was absent in most cases. Even more variations of K19 expression were observed in SSC. These findings indicate differences in the control of expression of K14 and K19 ill normal epithelia and show that regulation is further disturbed during dysplastic change and malignancy.
引用
收藏
页码:293 / 301
页数:9
相关论文
共 42 条
[1]   CONCERTED GENE DUPLICATIONS IN THE 2 KERATIN GENE FAMILIES [J].
BLUMENBERG, M .
JOURNAL OF MOLECULAR EVOLUTION, 1988, 27 (03) :203-211
[2]   EXTENSIVE CHANGES IN CYTOKERATIN EXPRESSION PATTERNS IN PATHOLOGICALLY AFFECTED HUMAN GINGIVA [J].
BOSCH, FX ;
OUHAYOUN, JP ;
BADER, BL ;
COLLIN, C ;
GRUND, C ;
LEE, I ;
FRANKE, WW .
VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY, 1989, 58 (01) :59-77
[3]   MESENCHYME-MEDIATED AND ENDOGENOUS REGULATION OF GROWTH AND DIFFERENTIATION OF HUMAN SKIN KERATINOCYTES DERIVED FROM DIFFERENT BODY SITES [J].
BOUKAMP, P ;
BREITKREUTZ, D ;
STARK, HJ ;
FUSENIG, NE .
DIFFERENTIATION, 1990, 44 (02) :150-161
[4]   COMBINING CONFOCAL AND CONVENTIONAL MODES IN TANDEM SCANNING REFLECTED LIGHT-MICROSCOPY [J].
BOYDE, A .
SCANNING, 1989, 11 (03) :147-152
[5]   TURNOVER OF CYTOKERATIN POLYPEPTIDES IN MOUSE HEPATOCYTES [J].
DENK, H ;
LACKINGER, E ;
ZATLOUKAL, K ;
FRANKE, WW .
EXPERIMENTAL CELL RESEARCH, 1987, 173 (01) :137-143
[6]   GENETIC SKIN DISORDERS OF KERATIN [J].
FUCHS, E .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1992, 99 (06) :671-674
[7]   THE CDNA SEQUENCE OF A HUMAN EPIDERMAL KERATIN - DIVERGENCE OF SEQUENCE BUT CONSERVATION OF STRUCTURE AMONG INTERMEDIATE FILAMENT PROTEINS [J].
HANUKOGLU, I ;
FUCHS, E .
CELL, 1982, 31 (01) :243-252
[8]   ACETYLATION OF CHROMOSOME SQUASHES OF DROSOPHILA-MELANOGASTER DECREASES BACKGROUND IN AUTORADIOGRAPHS FROM HYBRIDIZATION WITH [I125] LABELED RNA [J].
HAYASHI, S ;
GILLAM, IC ;
DELANEY, AD ;
TENER, GM .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1978, 26 (08) :677-679
[9]   CYTOKERATINS AS EPITHELIAL DIFFERENTIATION MARKERS IN PREMALIGNANT AND MALIGNANT ORAL LESIONS [J].
HEYDEN, A ;
HUITFELDT, HS ;
KOPPANG, HS ;
THRANE, PS ;
BRYNE, M ;
BRANDTZAEG, P .
JOURNAL OF ORAL PATHOLOGY & MEDICINE, 1992, 21 (01) :7-11
[10]  
KRAMER IRH, 1980, ORAL PREMALIGNANCY, P23