Occurrence of Virulence and Antimicrobial Resistance Determinants in Vibrio harveyi Isolated from Marine Food Fish Cultured in Korea

被引:6
作者
De Silva, Liyana Arachchilage Dinithi S.
Wickramanayake, Masimbule Vidanalage Kasun Sameera
Heo, Gang-Joon [1 ,2 ]
机构
[1] Chungbuk Natl Univ, Lab Aquat Anim Med, Ctr Vet Med, Chungdae Ro 1, Cheongju 28644, Chungbuk, South Korea
[2] Chungbuk Natl Univ, Coll Vet Med, Chungdae Ro 1, Cheongju 28644, Chungbuk, South Korea
关键词
Vibrio harveyi; virulence factors; antimicrobial resistance; seafood; MULTIPLEX PCR; PARAHAEMOLYTICUS STRAINS; MOLECULAR CHARACTERIZATION; ANTIBIOTIC-RESISTANCE; TOXR GENE; IN-VITRO; PATHOGENICITY; CHOLERAE; IDENTIFICATION; AQUACULTURE;
D O I
10.1089/mdr.2020.0618
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Vibrio harveyi is a significant cause of infection in both marine animals and humans. It has been reported frequently in seafood-borne infections worldwide. This study was conducted to determine the potential health impact of the V. harveyi isolated from marine food fish cultured in Korea concerning their virulence and antimicrobial resistance. A total of 49 V. harveyi samples were isolated by biochemical tests and multiplex PCR. Phenotypic detection of virulence factors resulted DNase activity (81.63%), hemolysis (alpha = 75.51% and beta = 12.25), gelatinase activity (71.43%), protease production (71.43%), phospholipase activity (65.31%), and lipase production (34.69%). Virulence genes, including VPI, tlh, tdh, toxR, VAC, and ctxAB, were detected in 57.14%, 44.90%, 36.73%, 22.45%, 12.24%, and 8.16% of the isolates, respectively. Resistance to ampicillin (77.55%), oxacillin (69.39%), nalidixic acid (53.06%), amoxicillin (46.94%), oxytetracycline (46.94%), colistin sulfate (34.69%), fosfomycin (34.69%), chloramphenicol (32.65%), streptomycin (32.65%), cephalothin (28.57%), oxytetracycline (26.53%), ceftriaxone (20.41%), erythromycin (14.29%), and cefoxitin (12.24%) was detected in disc diffusion assay. Most of the isolates were classified as multidrug resistant as they scored multiple antimicrobial resistance index >= 0.2. Furthermore, antimicrobial resistance genes tetB, qnrA, intI1 (Class 1 integron integrase), aac(6 ')-Ib, bla(SHV), bla(CTX-M), strA-strB, tetA, aphAI-IAB, qnrC, qnrS, and bla(TEM) were found in 81.63%, 67.35%, 61.22%, 46.94%, 44.90%, 44.90%, 36.73%, 18.37%, 10.20%, 10.20%, 8.16% and 6.12% of the isolates, respectively. In conclusion, the development of antimicrobial resistance among V. harveyi will ultimately reduce the efficacy of antimicrobials used for treating and can favor the development of more virulent V. harveyi strains.
引用
收藏
页码:255 / 265
页数:11
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