Surface plasmon resonance analysis at a supported lipid monolayer

被引：113
作者：
Cooper, MA
Try, AC
Carroll, J
Ellar, DJ
Williams, DH
机构：
[1] Univ Cambridge, Cambridge Ctr Mol Recognit, Dept Chem, Cambridge CB2 1EW, England
[2] Univ Cambridge, Cambridge Ctr Mol Recognit, Dept Biochem, Cambridge CB2 1GA, England
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1998年 / 1373卷 / 01期
基金：
英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;
关键词：
surface plasmon resonance; lipid monolayer; kinetics; glycopeptide antibiotic; glycosylphosphatidylinositol; aminopeptidase N; Cry1Ac; toxin;
D O I：
10.1016/S0005-2736(98)00091-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Methods for the formation of supported lipid monolayers on top of a hydrophobic self assembled monolayer in a surface plasmon resonance instrument are described. Small unilamellar vesicles absorb spontaneously to the surface of the hydrophobic self-assembled monolayer to form a surface which resembles the surface of a cellular membrane, Lipophilic ligands, such as small acylated peptides or glycosylphosphatidylinositol-anchored proteins, were inserted into the absorbed lipid and binding of analytes to these ligands was analysed by surface plasmon resonance. Conditions for the formation of lipid monolayers have been optimised with respect to lipid type, chemical and buffer compatibility, ligand stability and reproducibility. (C) 1998 Elsevier Science B.V. All rights reserved.
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页码：101 / 111
页数：11
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