Isolation and characterization of anti-diabetic compound from Clerodendrum infortunatum L. leaves

Clerodendrum infortunatum L. (CI) leaves are traditionally used in the treatment of diabetic patients. This study aimed to isolate and characterize bioactive compounds from CI leaves and evaluate their anti-diabetic activities (ADI). The CI leaves extracts ADI was estimated on streptozotocin-induced hyperglycemic rats by applying a different dose of extract and compared with the anti-diabetic medicine (Glibenclamide). The intraperitoneal's acute effect showed the progressive fall of the blood glucose level until 120 min on diabetic rats by applying the CI extract. Continuous administration of the target ethanolic extracts of CI at 0 to 28 days leads to a decrease in fasting serum glucose (FSG), total cholesterol (TG), and low-density lipoprotein (LDL) levels, while high-density lipoprotein (HDL) was increased. The ethanolic extract of CI produced five fractions, fraction-1 indicated a greater insulin secretion effect compared to other fractions. So, the structure of fraction-1 was elucidated and characterized by IR, H-1, C-13-NMR, COSY, and MS and the identified compound name is phaeophytin a. This study suggests that phaeophytin a exhibits significant anti-diabetic activity. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.