Berberine modulates cisplatin sensitivity of human gastric cancer cells by upregulation of miR-203

被引：56
作者：
You, He-Yi [1 ]
Xie, Xue-Meng [1 ]
Zhang, Wei-Jian [1 ]
Zhu, Heng-Liang [1 ]
Jiang, Fei-Zhao [1 ]
机构：
[1] Wenzhou Med Univ, Affiliated Hosp 1, Dept Laparoscop Surg, Wenzhou 325000, Peoples R China
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2016年 / 52卷 / 08期
关键词：
Berberine; MicroRNA-203; Apoptosis; Bcl-w; Gastric cancer; HEPATOCELLULAR-CARCINOMA; SIGNALING PATHWAY; INDUCED APOPTOSIS; DOWN-REGULATION; EXPRESSION; MICRORNAS; INCREASES; GROWTH; STAT3;
D O I：
10.1007/s11626-016-0044-y
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Chemotherapeutic resistance is the main reason of the failure in clinical treatment of gastric cancer. Berberine (BER) is the active compound of traditional Chinese medicine Huang Lian. The aim of this present study is to evaluate the effect of BER on cisplatin resistance in gastric cancer cells and to investigate its possible mechanism. Gastric cancer cell lines SGC-7901 and BGC-823 and their respective cisplatin-resistant variants SGC-7901/DDP and BGC-823/DDP were used in this study. We found that BER treatment significantly reversed cisplatin sensitivity and induced caspase-dependent apoptosis in SGC-7901/DDP and BGC-823/DDP cells; BER treatment induced miR-203 expression, and overexpression of miR-203 mimicked the cisplatin-sensitizing effect of BER. Importantly, we showed that miR-203 was able to target the 3'UTR of Bcl-w. Therefore, we conclude that BER treatment reduces cisplatin resistance of gastric cancer cells by modulating the miR-203/Bcl-w apoptotic axis. BER may be a novel agent to enhance chemotherapeutic responses in cisplatin-resistant gastric cancer patients.
引用
收藏
页码：857 / 863
页数：7
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