Glycoproteins play an important role in biological processes, such as cell recognition, cell adhesion, immunogenic recognition, and so on. Additionally, the carbohydrate moieties of the glycoprotein contribute to the solubility and thermal stability of proteins and to protection against proteolysis (1). In order to study these mechanisms, preparation of glycoproteins is required. In this review, preparation of glycopeptide using conventional chemical and recent manners (chemo-enzymatic method, using of natural source) is described.