An organization's long-term survival depends upon its capability to have a balance of exploitation of existing technologies with the exploration of future opportunities. This balanced approach of working is termed as Organization Ambidexterity (OA). This paper attempts to explain, empirically, the role of different organizational factors on OA and to compare results for manufacturing private and public sector firms in the Indian context (sample size n = 120). OA antecedents like strategic orientation, top management, manager, HR policies, organization culture, individual characteristics, organization structure, procedures & policies, and informal routines have been identified and hypotheses are presented for OA as the dependent variable. To validate hypotheses, a questionnaire survey was conducted. Results of multiple regression analysis and correlation are demonstrated to illustrate the dependence of OA on these organizational factors. Analysis shows that these variables have a different impact on OA in the public and private sectors.