FoxF is essential for FGF- induced migration of heart progenitor cells in the ascidian Ciona intestinalis

被引：80

作者：

Beh, Jeni ^{[1
]}

Shi, Weiyang ^{[1
]}

Levine, Mike ^{[1
]}

Davidson, Brad ^{[1
]}

Christiaen, Lionel ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Ctr Integrat Genom, Berkeley, CA 94720 USA

来源：

DEVELOPMENT | 2007年 / 134卷 / 18期

关键词：

D O I：

10.1242/dev.010140

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Heart development requires precise coordination of morphogenetic movements with progressive cell fate specification and differentiation. In ascidian embryos, FGF/MAPK-mediated activation of the transcription factor Ets1/2 is required for heart tissue specification and cell migration. We found that FoxF is one of the first genes to be activated in heart precursors in response to FGF signaling. We identified the FoxF minimal heart enhancer and used a cis-trans complementation test to show that Ets1/2 can interact with the FoxF enhancer in vivo. Next, we found that FoxF function is required downstream and in parallel to the FGF/MAPK/Ets cascade for cell migration. In addition, we demonstrated that targeted expression of a dominant-negative form of FoxF inhibits cell migration but not heart differentiation, resulting in a striking phenotype: a beating heart at an ectopic location within the body cavity of juveniles. Taken together, our results indicate that FoxF is a direct target of FGF signaling and is predominantly involved in the regulation of heart cell migration.

引用

页码：3297 / 3305

页数：9

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