The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger

被引：27

作者：

Mojzita, Dominik ^{[1
]}

Vuoristo, Kiira ^{[1
]}

Koivistoinen, Outi M. ^{[1
]}

Penttila, Merja ^{[1
]}

Richard, Peter ^{[1
]}

机构：

[1] VTT, Tech Res Ctr Finland, Espoo 02044, Finland

来源：

FEBS LETTERS | 2010年 / 584卷 / 16期

基金：

芬兰科学院;

关键词：

EC; 1.1.1.10; L-Arabinose metabolism; L-Xylulose; Pentose; Aspergillus niger; L-ARABINOSE METABOLISM; HYPOCREA-JECORINA; CATABOLIC PATHWAY; GENE; DEHYDROGENASE; EXPRESSION; CLONING; PENTOSE; YEAST;

D O I：

10.1016/j.febslet.2010.06.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

L-Xylulose reductase is part of the eukaryotic pathway for L-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on L-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3540 / 3544

页数：5

共 25 条

[1] Cloning and expression of a xylitol-4-dehydrogenase gene from Pantoea ananatis [J].