Simultaneous electrochemical determination of uric acid, xanthine and hypoxanthine based on poly(L-arginine)/graphene composite film modified electrode

被引：133

作者：

Zhang, Fengyuan ^{[1
]}

Wang, Zaihua ^{[1
]}

Zhang, Yuzhen ^{[1
]}

Zheng, Zhixiang ^{[1
]}

Wang, Chunming ^{[1
]}

Du, Yongling ^{[1
]}

Ye, Weichun ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

来源：

TALANTA | 2012年 / 93卷

关键词：

Graphene; Poly(L-arginine); Uric acid; Xanthine; Hypoxanthine; PERFORMANCE LIQUID-CHROMATOGRAPHY; CARBON-PASTE ELECTRODE; EXFOLIATED GRAPHITE OXIDE; PURINE COMPOUNDS; GRAPHENE SHEETS; NANOSHEETS; ALLANTOIN; ELECTROPHORESIS; QUANTIFICATION; NANOPARTICLE;

D O I：

10.1016/j.talanta.2012.02.041

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Poly(L-arginine)/graphene composite film modified electrode was successfully prepared via a facile onestep electrochemical method and used for simultaneous determination of uric acid (UA), xanthine (XA) and hypoxanthine (HX). The electrochemical behaviors of UA, XA and HX at the modified electrode were studied by cyclic voltammetry and differential pulse voltammetry (DPV), and showed that the modified electrode exhibited excellent electrocatalytic activity toward the oxidation of the three compounds. The calibration curves for UA, XA and HX were obtained over the range of 0.10-10.0, 0.10-10.0 and 0.20-20.0 mu M by DPV, respectively and the detection limits for UA, XA and HX were 0.05, 0.05 and 0.10 mu M (S/N = 3), respectively. With good selectivity and high sensitivity, the modified electrode has been applied to simultaneous determination of UA. XA and HX in human urine with satisfactory result. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：320 / 325

页数：6

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