Co-immunization of DNA and Protein in the Same Anatomical Sites Induces Superior Protective Immune Responses against SHIV Challenge

被引：44

作者：

Felber, Barbara K. ^{[1
]}

Lu, Zhongyan ^{[1
]}

Hu, Xintao ^{[1
]}

Valentin, Antonio ^{[2
]}

Rosati, Margherita ^{[2
]}

Remmel, Christopher A. L. ^{[3
]}

Weiner, Joshua A. ^{[3
]}

Carpenter, Margaret C. ^{[3
]}

Faircloth, Katelyn ^{[3
]}

Stanfield-Oakley, Sherry ^{[4
,5
,6
]}

Williams, Wilton B. ^{[4
]}

Shen, Xiaoying ^{[4
]}

Tomaras, Georgia D. ^{[4
,5
,6
,7
]}

LaBranche, Celia C. ^{[4
,5
]}

Montefiori, David ^{[4
,5
]}

Trinh, Hung, V ^{[8
,9
]}

Rao, Mangala ^{[8
]}

Alam, Munir S. ^{[4
]}

Vandergrift, Nathan A. ^{[4
,19
]}

Saunders, Kevin O. ^{[4
,5
]}

Wang, Yunfei ^{[4
]}

Rountree, Wes ^{[4
]}

Das, Jishnu ^{[10
]}

Alter, Galit ^{[10
]}

Reed, Steven G. ^{[11
]}

Aye, Pyone P. ^{[12
]}

Schiro, Faith ^{[12
]}

Pahar, Bapi ^{[12
]}

Dufour, Jason P. ^{[12
]}

Veazey, Ronald S. ^{[12
]}

Marx, Preston A. ^{[13
,14
]}

Venzon, David J. ^{[15
]}

Shaw, George M. ^{[16
,17
]}

Ferrari, Guido ^{[4
,5
,6
]}

Ackerman, Margaret E. ^{[3
]}

Haynes, Barton F. ^{[4
,18
]}

Pavlakis, George N. ^{[2
]}

机构：

[1] NCI Frederick, Human Retrovirus Pathogenesis Sect, Vaccine Branch, Ctr Canc Res, Frederick, MD 21702 USA

[2] NCI Frederick, Human Retrovirus Sect, Vaccine Branch, Ctr Canc Res, Frederick, MD 21702 USA

[3] Dartmouth Coll, Thayer Sch Engn, Hanover, NH 03755 USA

[4] Duke Univ, Duke Human Vaccine Inst, Durham, NC 27710 USA

[5] Duke Univ, Dept Surg, Durham, NC 27710 USA

[6] Duke Univ, Dept Mol Genet & Microbiol, Durham, NC 27710 USA

[7] Duke Univ, Dept Immunol, Durham, NC 27710 USA

[8] US Mil HIV Res Program, Walter Reed Army Inst Res, Silver Spring, MD 20910 USA

[9] Henry M Jackson Fdn Adv Mil Med, Bethesda, MD 20817 USA

[10] MIT & Harvard, Ragon Inst MGH, Cambridge, MA 02139 USA

[11] Infect Dis Res Inst, Seattle, WA 98102 USA

[12] Tulane Natl Primate Res Ctr, Div Comparat Pathol, Covington, LA 70433 USA

[13] Tulane Univ, Sch Publ Hlth & Trop Med, Tulane Natl Primate Res Ctr, New Orleans, LA 70112 USA

[14] Tulane Univ, Sch Publ Hlth & Trop Med, Dept Trop Med, New Orleans, LA 70112 USA

[15] NCI, Biostat & Data Management Sect, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[16] Univ Penn, Dept Med, Philadelphia, PA 19104 USA

[17] Univ Penn, Dept Microbiol, Philadelphia, PA 19104 USA

[18] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA

[19] RTI Int, 3040 Cornwallis Rd, Res Triangle Pk, NC 27709 USA

来源：

CELL REPORTS | 2020年 / 31卷 / 06期

基金：

美国国家卫生研究院;

关键词：

SIMIAN IMMUNODEFICIENCY VIRUS; NEUTRALIZING ANTIBODY-RESPONSES; HIV-1 VACCINE EFFICACY; FUSION INHIBITOR T-20; CHRONIC VIREMIA; CELL RESPONSES; SIV CHALLENGES; ENVELOPE; EPITOPE; TYPE-1;

D O I：

10.1016/j.celrep.2020.107624

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We compare immunogenicity and protective efficacy of an HIV vaccine comprised of env and gag DNA and Env (Envelope) proteins by co-administration of the vaccine components in the same muscles or by separate administration of DNA + protein in contralateral sites in female rhesus macaques. The 6-valent vaccine includes gp145 Env DNAs, representing six sequentially isolated Envs from the HIV-infected individual CH505, and matching GLA-SE-adjuvanted gp120 Env proteins. Interestingly, only macaques in the co-administration vaccine group are protected against SHIV CH505 acquisition after repeated low-dose intravaginal challenge and show 67% risk reduction per exposure. Macaques in the co-administration group develop higher Env-specific humoral and cellular immune responses. Non-neutralizing Env antibodies, ADCC, and antibodies binding to Fc gamma RIIIa are associated with decreased transmission risk. These data suggest that simultaneous recognition, processing, and presentation of DNA + Env protein in the same draining lymph nodes play a critical role in the development of protective immunity.

引用

页数：22

共 91 条

[1] Route of immunization defines multiple mechanisms of vaccine-mediated protection against SIV [J].

Ackerman, Margaret E. ;

Das, Jishnu ;

Pittala, Srivamshi ;

Broge, Thomas ;

Linde, Caitlyn ;

Suscovich, Todd J. ;

Brown, Eric P. ;

Bradley, Todd ;

Natarajan, Harini ;

Lin, Shu ;

Sassic, Jessica K. ;

O'Keefe, Sean ;

Mehta, Nickita ;

Goodman, Derrick ;

Sips, Magdalena ;

Weiner, Joshua A. ;

Tomaras, Georgia D. ;

Haynes, Barton F. ;

Lauffenburger, Douglas A. ;

Bailey-Kellogg, Chris ;

Roederer, Mario ;

Alter, Galit .

NATURE MEDICINE, 2018, 24 (10) :1590-+

[2] Systems serology for evaluation of HIV vaccine trials [J].

Ackerman, Margaret E. ;

Barouch, Dan H. ;

Alter, Galit .

IMMUNOLOGICAL REVIEWS, 2017, 275 (01) :262-270

[3] Immune Correlate-Guided HIV Vaccine Design [J].

Alter, Galit ;

Barouch, Dan .

CELL HOST & MICROBE, 2018, 24 (01) :25-33

[4]

Barouch DH, 2018, LANCET, V392, P232, DOI [10.1016/S0140-6736(18)31364-3, 10.1016/s0140-6736(18)31364-3]

[5] Protective efficacy of adenovirus/protein vaccines against SIV challenges in rhesus monkeys [J].

Barouch, Dan H. ;

Alter, Galit ;

Broge, Thomas ;

Linde, Caitlyn ;

Ackerman, Margaret E. ;

Brown, Eric P. ;

Borducchi, Erica N. ;

Smith, Kaitlin M. ;

Nkolola, Joseph P. ;

Liu, Jinyan ;

Shields, Jennifer ;

Parenteau, Lily ;

Whitney, James B. ;

Abbink, Peter ;

Ng'ang'a, David M. ;

Seaman, Michael S. ;

Lavine, Christy L. ;

Perry, James R. ;

Li, Wenjun ;

Colantonio, Arnaud D. ;

Lewis, Mark G. ;

Chen, Bing ;

Wenschuh, Holger ;

Reimer, Ulf ;

Piatak, Michael ;

Lifson, Jeffrey D. ;

Handley, Scott A. ;

Virgin, Herbert W. ;

Koutsoukos, Marguerite ;

Lorin, Clarisse ;

Voss, Gerald ;

Weijtens, Mo ;

Pau, Maria G. ;

Schuitemaker, Hanneke .

SCIENCE, 2015, 349 (6245) :320-324

[6] Vaccine protection against acquisition of neutralization-resistant SIV challenges in rhesus monkeys [J].

Barouch, Dan H. ;

Liu, Jinyan ;

Li, Hualin ;

Maxfield, Lori F. ;

Abbink, Peter ;

Lynch, Diana M. ;

Iampietro, M. Justin ;

SanMiguel, Adam ;

Seaman, Michael S. ;

Ferrari, Guido ;

Forthal, Donald N. ;

Ourmanov, Ilnour ;

Hirsch, Vanessa M. ;

Carville, Angela ;

Mansfield, Keith G. ;

Stablein, Donald ;

Pau, Maria G. ;

Schuitemaker, Hanneke ;

Sadoff, Jerald C. ;

Billings, Erik A. ;

Rao, Mangala ;

Robb, Merlin L. ;

Kim, Jerome H. ;

Marovich, Mary A. ;

Goudsmit, Jaap ;

Michael, Nelson L. .

NATURE, 2012, 482 (7383) :89-U115

[7] Antibody-virus co-evolution in HIV infection: paths for HIV vaccine development [J].

Bonsignori, Mattia ;

Liao, Hua-Xin ;

Gao, Feng ;

Williams, Wilton B. ;

Alam, S. Munir ;

Montefiori, David C. ;

Haynes, Barton F. .

IMMUNOLOGICAL REVIEWS, 2017, 275 (01) :145-160

[8] Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody [J].

Bonsignori, Mattia ;

Zhou, Tongqing ;

Sheng, Zizhang ;

Chen, Lei ;

Gao, Feng ;

Joyce, M. Gordon ;

Ozorowski, Gabriel ;

Chuang, Gwo-Yu ;

Schramm, Chaim A. ;

Wiehe, Kevin ;

Alam, S. Munir ;

Bradley, Todd ;

Gladden, Morgan A. ;

Hwang, Kwan-Ki ;

Iyengar, Sheelah ;

Kumar, Amit ;

Lu, Xiaozhi ;

Luo, Kan ;

Mangiapani, Michael C. ;

Parks, Robert J. ;

Song, Hongshuo ;

Acharya, Priyamvada ;

Bailer, Robert T. ;

Cao, Allen ;

Druz, Aliaksandr ;

Georgiev, Ivelin S. ;

Kwon, Young D. ;

Louder, Mark K. ;

Zhang, Baoshan ;

Zheng, Anqi ;

Hill, Brenna J. ;

Kong, Rui ;

Soto, Cinque ;

Mullikin, James C. ;

Douek, Daniel C. ;

Montefiori, David C. ;

Moody, Michael A. ;

Shaw, George M. ;

Hahn, Beatrice H. ;

Kelsoe, Garnett ;

Hraber, Peter T. ;

Korber, Bette T. ;

Boyd, Scott D. ;

Fire, Andrew Z. ;

Kepler, Thomas B. ;

Shapiro, Lawrence ;

Ward, Andrew B. ;

Mascola, John R. ;

Liao, Hua-Xin ;

Kwong, Peter D. .

CELL, 2016, 165 (02) :449-463

[9] Analysis of a Clonal Lineage of HIV-1 Envelope V2/V3 Conformational Epitope-Specific Broadly Neutralizing Antibodies and Their Inferred Unmutated Common Ancestors [J].

Bonsignori, Mattia ;

Hwang, Kwan-Ki ;

Chen, Xi ;

Tsao, Chun-Yen ;

Morris, Lynn ;

Gray, Elin ;

Marshall, Dawn J. ;

Crump, John A. ;

Kapiga, Saidi H. ;

Sam, Noel E. ;

Sinangil, Faruk ;

Pancera, Marie ;

Yang Yongping ;

Zhang, Baoshan ;

Zhu, Jiang ;

Kwong, Peter D. ;

O'Dell, Sijy ;

Mascola, John R. ;

Wu, Lan ;

Nabel, Gary J. ;

Phogat, Sanjay ;

Seaman, Michael S. ;

Whitesides, John F. ;

Moody, M. Anthony ;

Kelsoe, Garnett ;

Yang, Xinzhen ;

Sodroski, Joseph ;

Shaw, George M. ;

Montefiori, David C. ;

Kepler, Thomas B. ;

Tomaras, Georgia D. ;

Alam, S. Munir ;

Liao, Hua-Xin ;

Haynes, Barton F. .

JOURNAL OF VIROLOGY, 2011, 85 (19) :9998-10009

[10] Pentavalent HIV-1 vaccine protects against simian-human immunodeficiency virus challenge [J].

Bradley, Todd ;

Pollara, Justin ;

Santra, Sampa ;

Vandergrift, Nathan ;

Pittala, Srivamshi ;

Bailey-Kellogg, Chris ;

Shen, Xiaoying ;

Parks, Robert ;

Goodman, Derrick ;

Eaton, Amanda ;

Balachandran, Harikrishnan ;

Mach, Linh V. ;

Saunders, Kevin O. ;

Weiner, Joshua A. ;

Scearce, Richard ;

Sutherland, Laura L. ;

Phogat, Sanjay ;

Tartaglia, Jim ;

Reed, Steven G. ;

Hu, Shiu-Lok ;

Theis, James F. ;

Pinter, Abraham ;

Montefiori, David C. ;

Kepler, Thomas B. ;

Peachman, Kristina K. ;

Rao, Mangala ;

Michael, Nelson L. ;

Suscovich, Todd J. ;

Alter, Galit ;

Ackerman, Margaret E. ;

Moody, M. Anthony ;

Liao, Hua-Xin ;

Tomaras, Georgia ;

Ferrari, Guido ;

Korber, Bette T. ;

Haynes, Barton F. .

NATURE COMMUNICATIONS, 2017, 8

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