Rosiglitazone-mediated dendritic cells ameliorate collagen-induced arthritis in mice

被引：16

作者：

Byun, Sei-Hee ^{[1
]}

Lee, Jun-Ho ^{[1
,2
]}

Jung, Nam-Chul ^{[2
]}

Choi, Hyun-Ji ^{[1
]}

Song, Jie-Young ^{[3
]}

Seo, Han Geuk ^{[4
]}

Choi, Jinjung ^{[5
]}

Jung, Sang Youn ^{[5
]}

Kang, Sangjin ^{[1
]}

Choi, Yong-Soo ^{[1
]}

Chung, Ji Hyung ^{[1
]}

Lim, Dae-Seog ^{[1
]}

机构：

[1] CHA Univ, Dept Biotechnol, 335 Pangyo Ro, Songnam 463400, Gyeonggi Do, South Korea

[2] Pharos Vaccine Inc, 545 Dunchon Daero, Songnam 462807, Gyeonggi Do, South Korea

[3] Korea Inst Radiol & Med Sci, Dept Radiat Canc Sci, 215-4 Gongneung Dong, Seoul 139706, South Korea

[4] Konkuk Univ, Dept Anim Biotechnol, 1 Hwayang Dong, Seoul 143701, South Korea

[5] Bundang CHA Med Ctr, Div Rheumatol, Songnam 463712, Gyeonggi Do, South Korea

来源：

BIOCHEMICAL PHARMACOLOGY | 2016年 / 115卷

关键词：

Autoimmune diseases; Dendritic cells; Treg cells; Mouse models RA; REGULATORY T-CELLS; RHEUMATOID-ARTHRITIS; PPAR-GAMMA; AUTOIMMUNE ARTHRITIS; PERIPHERAL-BLOOD; IN-VIVO; ACTIVATION; MACROPHAGE; RECEPTORS; AGONISTS;

D O I：

10.1016/j.bcp.2016.05.009

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Rosiglitazone is a selective ligand for peroxisome proliferator-activated receptor-gamma (PPAR-gamma), which serves diverse biological functions. A number of autoimmune disease models have been used to examine the anti-inflammatory and immunosuppressive effects of tolerogenic dendritic cells (tDCs). The aim of the present study was to investigate whether rosiglitazone-mediated DC (Rosi-DC) therapy suppressed arthritis in a collagen-induced arthritis (CIA) mouse model. Rosi-DCs were generated by treating immature DCs with TNF-alpha, type II collagen, and rosiglitazone. CIA mice then received subcutaneously (s.c.) two injections of Rosi-DCs. The severity of arthritis was then assessed histopathologically. The phenotypes of the DC and regulatory T (Treg) cell populations in CIA mice were determined by flow cytometry and the effect of Rosi-DCs on the secretion of autoimmunity-inducing cytokines was examined by ELISA. Rosi-DCs expressed lower levels of DC-related surface markers than mature DCs. Histopathological examination revealed that the degree of inflammation in the paws of Rosi-DC-treated mice was much lower than that in the paws of PBS-treated CIA mice. Taken together, these results clearly show that rosiglitazone-mediated DCs ameliorate CIA, most likely via the induction of antigen-specific Treg cells. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：85 / 93

页数：9

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