RNA-guided genetic silencing systems in bacteria and archaea

被引：1435

作者：

Wiedenheft, Blake ^{[1
,2
]}

Sternberg, Samuel H. ^{[3
]}

Doudna, Jennifer A. ^{[1
,2
,3
,4
]}

机构：

[1] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[4] Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

NATURE | 2012年 / 482卷 / 7385期

基金：

美国国家科学基金会;

关键词：

PROVIDES ACQUIRED-RESISTANCE; CRISPR RNA; IMMUNE-SYSTEM; TARGET RECOGNITION; ANTIVIRAL DEFENSE; ESCHERICHIA-COLI; STRUCTURAL BASIS; REPEATS CRISPRS; DNA; IDENTIFICATION;

D O I：

10.1038/nature10886

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Clustered regularly interspaced short palindromic repeat (CRISPR) are essential components of nucleic-acid-based adaptive immune systems that are widespread in bacteria and archaea. Similar to RNA interference (RNAi) pathways in eukaryotes, CRISPR-mediated immune systems rely on small RNAs for sequence-specific detection and silencing of foreign nucleic acids, including viruses and plasmids. However, the mechanism of RNA-based bacterial immunity is distinct from RNAi. Understanding how small RNAs are used to find and destroy foreign nucleic acids will provide new insights into the diverse mechanisms of RNA-controlled genetic silencing systems.

引用

页码：331 / 338

页数：8

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