Pokeweed Antiviral Protein: Its Cytotoxicity Mechanism and Applications in Plant Disease Resistance

被引：36
作者：
Di, Rong [1 ]
Tumer, Nilgun E. [1 ]
机构：
[1] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA
来源：
TOXINS | 2015年 / 7卷 / 03期
关键词：
pokeweed antiviral protein; ribosome inactivating protein; rRNA depurination; apoptosis; ribosomal protein; broad spectrum disease resistance; RIBOSOME-INACTIVATING PROTEINS; RICIN A-CHAIN; N-GLYCOSIDASE ACTIVITY; RAY CRYSTALLOGRAPHIC ANALYSIS; ACTIVE-CENTER CLEFT; PHYTOLACCA-AMERICANA; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; MOSAIC-VIRUS; ESCHERICHIA-COLI;
D O I：
10.3390/toxins7030755
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Pokeweed antiviral protein (PAP) is a 29 kDa type I ribosome inactivating protein (RIP) found in pokeweed plants. Pokeweed produces different forms of PAP. This review focuses on the spring form of PAP isolated from Phytolacca americana leaves. PAP exerts its cytotoxicity by removing a specific adenine from the -sarcin/ricin loop of the large ribosomal RNA. Besides depurination of the rRNA, PAP has additional activities that contribute to its cytotoxicity. The mechanism of PAP cytotoxicity is summarized based on evidence from the analysis of transgenic plants and the yeast model system. PAP was initially found to be anti-viral when it was co-inoculated with plant viruses onto plants. Transgenic plants expressing PAP and non-toxic PAP mutants have displayed broad-spectrum resistance to both viral and fungal infection. The mechanism of PAP-induced disease resistance in transgenic plants is summarized.
引用
收藏
页码：755 / 772
页数：18
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