Chemical screening of sixty-one actinomycete strains and anti-methicillin-resistant Staphylococcus aureus assays of target strains

被引:5
作者
Yuan Gan-Jun [1 ]
Li Pei-Bo [2 ]
Yang Hui [1 ]
Wu Xiao-Yu [1 ]
Tu Guo-Quan [1 ]
Wei Sai-Jin [1 ]
机构
[1] Jiangxi Agr Univ, Coll Biosci & Engn, Nanchang 330045, Peoples R China
[2] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
关键词
Actinomycetes; Chemical screening; Anti-MRSA; Strain ZZ027; Strain ZZ021; STRUCTURE ELUCIDATION; THIAZOMYCIN;
D O I
10.3724/SP.J.1009.2012.00155
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
AIM: To discover actinomycete strains-producing analogs and structure-types with anti-methicillin-resistant Staphylococcus aureu (anti-MRSA) activity from a soil used as an anti-infection folk medicine. METHODS: Plate method was used for the isolation of actinomycete strains with an improved Gauss medium, and chemical screening of these strains was achieved with HPLC-UV technique. The anti-MRSA activities and minimum inhibitory concentrations (MICs) of the extracts of the cultures of target strains were performed by agar diffusion and broth microdilution respectively, and their cytotoxicity was evaluated by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) method using human colon tumor cell HCT-116. RESULTS: Sixty-one actinomycete strains were isolated from the soil sample collected in Zhuzhou County, Hunan, China. Based on the analogs and structure-types in the HPLC profiles of the extracts of their cultures, three strains ZZ027, ZZ021 and ZZ044 were targeted by chemical screening. The extract (500 mu g) of the culture of ZZ027 and ZZ021 showed anti-MRSA activity with inhibition zones of 29-35 and 17-24 mm respectively, and their MICs were 0.625-1.25 and 2.50 mg.mL(-1) respectively. The extract obtained from ZZ021 showed remarkable cytotoxicity against HCT-116 in vitro with IC50 value of 6.4 mu g.mL(-1), while those of ZZ027 and ZZ044 showed no cytotoxicity (IC50 values were more than 20 mu g.mL(-1)). CONCLUSION: From a soil sample used as an anti-infectious folk medicine, three strains Z2027, ZZ021 and ZZ044 were obtained by selective isolation and chemical screening. ZZ027 and ZZ021 showed anti-MRSA activities, and ZZ021 showed remarkable cytotoxicity.
引用
收藏
页码:155 / 160
页数:6
相关论文
共 12 条
[1]   Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens [J].
Ahmad, I ;
Beg, AZ .
JOURNAL OF ETHNOPHARMACOLOGY, 2001, 74 (02) :113-123
[2]  
[Anonymous], 2006, METH DIL ANT SUSC TE, Vseventh
[3]   Drug discovery from natural sources [J].
Chin, Young-Won ;
Balunas, Marcy J. ;
Chai, Hee Byung ;
Kinghorn, A. Douglas .
AAPS JOURNAL, 2006, 8 (02) :E239-E253
[4]   Targeted Isolation and Structure Elucidation of Stilbene Glycosides from the Bark of Lysidice brevicalyx Wei Guided by Biological and Chemical Screening [J].
Hu, Youcai ;
Ma, Shuanggang ;
Li, Jianbei ;
Yu, Shishan ;
Qu, Jing ;
Liu, Jing ;
Du, Dan .
JOURNAL OF NATURAL PRODUCTS, 2008, 71 (11) :1800-1805
[5]   Isolation and structure elucidation of thiazomycin -: A potent thiazolyl peptide antibiotic from Amycolatopsis fastidiosa [J].
Jayasuriya, Hiranthi ;
Herath, Kithsiri ;
Ondeyka, John G. ;
Zhang, Chaowei ;
Zink, Deborah L. ;
Brower, Mark ;
Gailliot, Francis P. ;
Greene, Joyce ;
Birdsall, Gwyneth ;
Venugopal, Jayashree ;
Ushio, Misti ;
Burgess, Bruce ;
Russotti, Greg ;
Walker, Andre ;
Hesse, Michelle ;
Seeley, Anna ;
Junker, Beth ;
Connors, Neal ;
Salazar, Oscar ;
Genilloud, Olga ;
Liu, Kun ;
Masurekar, Prakash ;
Barrett, John F. ;
Singh, Sheo B. .
JOURNAL OF ANTIBIOTICS, 2007, 60 (09) :554-564
[6]  
Jiyoung K, 2009, J MICROBIOL BIOTECHN, V19, P1
[7]   Chemical screening of olive biophenol extracts by hyphenated liquid chromatography [J].
Obied, Hassan K. ;
Bedgood, Danny R., Jr. ;
Prenzler, Paul D. ;
Robards, Kevin .
ANALYTICA CHIMICA ACTA, 2007, 603 (02) :176-189
[8]   Microbiology - Desperately seeking new antibiotics [J].
Payne, David J. .
SCIENCE, 2008, 321 (5896) :1644-1645
[9]   Reassessing the structure of pyranonigrin [J].
Schlingmann, Gerhard ;
Taniguchi, Tohru ;
He, Haiyin ;
Bigelis, Ramunas ;
Yang, Hui Y. ;
Koehn, Frank E. ;
Carter, Guy T. ;
Berova, Nina .
JOURNAL OF NATURAL PRODUCTS, 2007, 70 (07) :1180-1187
[10]  
Shaaban Mohamed, 2008, Bulletin of the National Research Centre (Cairo), V33, P311