Large-scale, high-resolution data acquisition system for extracellular recording of electrophysiological activity

被引：94

作者：

Imfeld, Kilian ^{[1
]}

Neukom, Simon ^{[2
]}

Maccione, Alessandro ^{[3
]}

Bornat, Yannick ^{[4
]}

Martinoia, Sergio ^{[3
]}

Farine, Pierre-Andre ^{[1
]}

Koudelka-Hep, Milerla ^{[5
]}

Berdondini, Luca ^{[5
]}

机构：

[1] Univ Neuchatel, Inst Microtechnol, Elect & Signal Proc Lab, CH-2000 Neuchatel, Switzerland

[2] CSEM, CH-8048 Zurich, Switzerland

[3] Univ Genoa, Dept Elect & Biomed Engn, I-16145 Genoa, Italy

[4] Univ Bordeaux, Lab Integrat Mat Syst IMS, F-33045 Bordeaux, France

[5] Univ Neuchatel, Inst Microtechnol, Sensors Actuators & Microsyst Lab, CH-2003 Neuchatel, Switzerland

来源：

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING | 2008年 / 55卷 / 08期

关键词：

active pixel sensor (APS); cardiomyocytes culture; electrophysiology; extracellular recording; high density; high spatiotemporal resolution; microelectrode array; real-time processing;

D O I：

10.1109/TBME.2008.919139

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A platform for high spatial and temporal resolution electrophysiological recordings of in vitro electrogenic cell cultures handling 4096 electrodes at a full frame rate of 8 kHz is presented and validated by means of cardiomyocyte cultures. Based on an active pixel sensor device implementing an array of metallic electrodes, the system provides acquisitions at spatial resolutions of 42 mu m on an active area of 2.67 mm x 2.67 mm, and in the zooming mode, temporal resolutions down to 8 mu s on 64 randomly selected electrodes. The low-noise performances of the integrated amplifier (11 mu V-rms) combined with a hardware implementation inspired by image/video processing concepts enable high-resolution acquisitions with real-time preprocessing capabilities adapted to the handling of the large amount of acquired data.

引用

页码：2064 / 2073

页数：10

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