Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation: an in vitro study

被引：7
作者：
Song, Q. [1 ,2 ]
Risco, R. [3 ]
Latina, M. [1 ,2 ]
Berthiaume, F. [1 ,2 ]
Nahmias, Y. [1 ,2 ]
Yarmush, M. L. [1 ,2 ]
机构：
[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Engn Med, Boston, MA 02114 USA
[2] Shriners Burns Hosp, Boston, MA 02114 USA
[3] Univ Seville, Escuela Super Ingn, Seville 41092, Spain
来源：
OPTICS EXPRESS | 2008年 / 16卷 / 14期
关键词：
D O I：
10.1364/OE.16.010518
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
This work describes the selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation. We observed: (1) single pulsed laser irradiation caused cellular damages on pigmented, and not on non-pigmented RPE cells at laser radiant exposure up to 2550 mJ/cm(2); (2) in the mixture of pigmented and non-pigmented RPE cells, single pulsed laser-induced damage was confined to pigmented RPE cells. This study demonstrates that the pigmented RPE cells can be selectively damaged, using a single pulsed laser irradiation, without thermal coagulation to adjacent non-pigmented RPE cells. (c) 2008 Optical Society of America.
引用
收藏
页码：10518 / 10528
页数：11
相关论文
共 50 条
[31]   CYTOKINE MODULATION OF INTEGRIN MOLECULE EXPRESSION ON RETINAL-PIGMENT EPITHELIAL (RPE) CELLS [J].
PLATTS, KE ;
BENSON, MT ;
REES, RC ;
RENNIE, IG .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1994, 35 (04) :2042-2042
[32]   MELATONIN INHIBITS THE PROLIFERATION OF RETINAL-PIGMENT EPITHELIAL (RPE) CELLS-INVITRO [J].
YU, HS ;
HERNANDEZ, V ;
HAYWOOD, M ;
WONG, CG .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 1993, 29A (05) :415-418
[33]   Melatonin protects human retinal pigment epithelial (RPE) cells against oxidative stress [J].
Liang, FQ ;
Green, L ;
Wang, C ;
Alssadi, R ;
Godley, BF .
EXPERIMENTAL EYE RESEARCH, 2004, 78 (06) :1069-1075
[34]   Identification of low-density lipofuscin in human retinal pigment epithelial (RPE) cells [J].
Wen, Rong ;
Guan, Ziqiang ;
Jiao, Shuliang ;
Lam, Byron L. ;
Li, Yiwen .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2016, 57 (12)
[35]   Electrophysiological Responses of Isolated Retinal Pigment Epithelial (RPE) Cells to Physiological Concentrations of Thiocyanate [J].
Hughes, Bret A. ;
Cao, Xu .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2019, 60 (09)
[36]   The lipofuscin component A2-E: Effects on retinal pigmented epithelial (RPE) cells in culture [J].
Sparrow, JR ;
Parish, C ;
Hashimoto, M ;
Liu, J ;
Nakanishi, K .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1999, 40 (04) :S927-S927
[37]   VARIATION IN PROTEOLYTIC ACTIVITY OF MACULAR AND PERIPHERAL RETINAL PIGMENTED EPITHELIAL (RPE) CELLS AS A FUNCTION OF AGE [J].
KARWATOWSKI, WSS ;
SMITH, EG ;
MACIEWICZ, RA .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1992, 33 (04) :909-909
[38]   Cellular and molecular responses to sublethal oxidative injury in retinal pigment epithelial cells (RPE) [J].
Strunnikova, NV ;
Baffi, J ;
Zhang, C ;
Teichberg, D ;
Becker, K ;
Cousins, SW ;
Csaky, KG .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2003, 44 :U125-U125
[39]   Lutein Supplementation Suppresses Inflammatory Responses in Retinal Pigment Epithelial Cells (RPE) and Macrophage [J].
Weikel, K. A. ;
Bian, Q. ;
Ren, Z. ;
Gao, S. ;
Qin, J. ;
Taylor, A. ;
Wu, D. ;
Tang, G. ;
Shang, F. .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2010, 51 (13)
[40]   In vitro Safety of Bromfenac on Human Retinal Pigment Epithelial Cells [J].
Patel, N. S. ;
Migon, R. ;
Mansoor, S. ;
Kenney, M. C. ;
Kuppermann, B. D. .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2010, 51 (13)
12345