Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation: an in vitro study

被引：7
|
作者：
Song, Q. [1 ,2 ]
Risco, R. [3 ]
Latina, M. [1 ,2 ]
Berthiaume, F. [1 ,2 ]
Nahmias, Y. [1 ,2 ]
Yarmush, M. L. [1 ,2 ]
机构：
[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Engn Med, Boston, MA 02114 USA
[2] Shriners Burns Hosp, Boston, MA 02114 USA
[3] Univ Seville, Escuela Super Ingn, Seville 41092, Spain
来源：
OPTICS EXPRESS | 2008年 / 16卷 / 14期
关键词：
D O I：
10.1364/OE.16.010518
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
This work describes the selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation. We observed: (1) single pulsed laser irradiation caused cellular damages on pigmented, and not on non-pigmented RPE cells at laser radiant exposure up to 2550 mJ/cm(2); (2) in the mixture of pigmented and non-pigmented RPE cells, single pulsed laser-induced damage was confined to pigmented RPE cells. This study demonstrates that the pigmented RPE cells can be selectively damaged, using a single pulsed laser irradiation, without thermal coagulation to adjacent non-pigmented RPE cells. (c) 2008 Optical Society of America.
引用
收藏
页码：10518 / 10528
页数：11
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