Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation: an in vitro study

被引：7
|
作者：
Song, Q. [1 ,2 ]
Risco, R. [3 ]
Latina, M. [1 ,2 ]
Berthiaume, F. [1 ,2 ]
Nahmias, Y. [1 ,2 ]
Yarmush, M. L. [1 ,2 ]
机构：
[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Engn Med, Boston, MA 02114 USA
[2] Shriners Burns Hosp, Boston, MA 02114 USA
[3] Univ Seville, Escuela Super Ingn, Seville 41092, Spain
来源：
OPTICS EXPRESS | 2008年 / 16卷 / 14期
关键词：
D O I：
10.1364/OE.16.010518
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
This work describes the selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation. We observed: (1) single pulsed laser irradiation caused cellular damages on pigmented, and not on non-pigmented RPE cells at laser radiant exposure up to 2550 mJ/cm(2); (2) in the mixture of pigmented and non-pigmented RPE cells, single pulsed laser-induced damage was confined to pigmented RPE cells. This study demonstrates that the pigmented RPE cells can be selectively damaged, using a single pulsed laser irradiation, without thermal coagulation to adjacent non-pigmented RPE cells. (c) 2008 Optical Society of America.
引用
收藏
页码：10518 / 10528
页数：11
相关论文
共 50 条
  • [1] RABBIT RETINAL PIGMENTED EPITHELIAL (RPE) CELLS IN CULTURE
    CLAFLIN, AJ
    CUZZANI, OE
    ABRAMS, GW
    IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION, 1980, 16 (03): : 212 - 213
  • [2] GROWTH-REGULATION OF RETINAL-PIGMENT EPITHELIAL (RPE) CELLS IN-VITRO
    KISHI, H
    MISHIMA, HK
    YAMASHITA, U
    CURRENT EYE RESEARCH, 1994, 13 (09) : 661 - 668
  • [3] Nanoscopic Approach to Study the Early Stages of Epithelial to Mesenchymal Transition (EMT) of Human Retinal Pigment Epithelial (RPE) Cells In Vitro
    Chtcheglova, Lilia A.
    Ohlmann, Andreas
    Boytsov, Danila
    Hinterdorfer, Peter
    Priglinger, Siegfried G.
    Priglinger, Claudia S.
    LIFE-BASEL, 2020, 10 (08): : 1 - 19
  • [4] Dopaminergic properties of retinal pigment epithelial (RPE) cells.
    Venkiteswaran, K
    Nelms, JL
    Tidwell, N
    Subramanian, T
    EXPERIMENTAL NEUROLOGY, 2002, 175 (02) : 446 - 446
  • [5] ISOLATION AND CULTIVATION OF RETINAL-PIGMENT EPITHELIAL (RPE) CELLS
    WEINSIEDER, A
    MCAULIFFE, WG
    RADTKE, ND
    CAPLE, S
    JOURNAL OF CELL BIOLOGY, 1983, 97 (05): : A465 - A465
  • [6] Glucose specific regulation of aldose reductase in human retinal pigment epithelial (RPE) cells in vitro
    Henry, DN
    Hootman, SR
    Rood, SE
    Busik, JV
    DIABETES, 1999, 48 : A157 - A157
  • [7] Temperature-dependent response of retinal pigment epithelial cells to laser irradiation
    Brinkmann, Ralf
    Iwami, Hisashi
    Pruessner, Joachim
    Danicke, Veit
    Miura, Yoko
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2013, 54 (15)
  • [8] A Method for the Isolation and Culture of Adult Rat Retinal Pigment Epithelial (RPE) Cells to Study Retinal Diseases
    Heller, Janosch P.
    Kwok, Jessica C. F.
    Vecino, Elena
    Martin, Keith R.
    Fawcett, James W.
    FRONTIERS IN CELLULAR NEUROSCIENCE, 2015, 9
  • [9] Apical sorting pathways in retinal pigment epithelial (RPE) cells.
    Bonilha, VL
    Marmorstein, AD
    Sparrow, JR
    RodriguezBoulan, E
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1996, 37 (03) : 1778 - 1778
  • [10] THE PRODUCTION OF THROMBOSPONDIN AND FIBRONECTIN BY RETINAL-PIGMENT EPITHELIAL (RPE) CELLS
    LARKIN, G
    HISCOTT, P
    SHERAIDAH, G
    OCCLESTON, NL
    KHAW, PT
    GRIERSON, I
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1994, 35 (04) : 2039 - 2039
12345