Detection and Quantification of Bovine Papilloma Virus Type 2 (BPV-2) by Real-time PCR in Urine and Urinary Bladder Lesions in Enzootic Bovine Haematuria (EBH)-Affected Cows

被引：21

作者：

Pathania, S. ^{[1
]}

Dhama, K. ^{[1
]}

Saikumar, G. ^{[1
]}

Shahi, S. ^{[1
]}

Somvanshi, R. ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Pathol, Izatnagar 243122, Uttar Pradesh, India

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2012年 / 59卷 / 01期

关键词：

bovine papillomavirus type 2; polymerase chain reaction; real-time PCR; urine; urinary bladder tumours; enzootic bovine haematuria; cows; EXPRESSION; SAMPLES; CANCER; CATTLE; DNA;

D O I：

10.1111/j.1865-1682.2011.01248.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

This study was conducted with the objectives of detecting bovine papillomavirus type 2 (BPV-2) in urine samples and urinary bladder lesions in bovines using polymerase chain reaction (PCR) and real-time PCR-based molecular diagnostic tests, and quantifying BPV-2 in urinary bladder lesions especially in enzootic bovine haematuria (EBH)-affected animals. BPV-2 viral DNA was detected in urine samples (50%) and urinary bladder tissue (68.6%). Cloning and sequencing results showed a close homology with other Indian BPV-2 sequences. Quantitative real-time PCR (SYBR Green assay) showed that the BPV-2 load was low and similar irrespective of inflammatory or neoplastic lesions in the bladder. It was concluded that BPV-2 DNA is frequently present in urine and urinary bladder lesions in cows in an EBH endemic region and virus load was low in urinary bladder lesions.

引用

页码：79 / 84

页数：6

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