In Vivo Analysis on the Adherence and Infection Route of Photobacterium damselae subsp piscicida in Yellowtail

被引:3
作者
Nagano, Ichiro [2 ]
Oshima, Syun-ichirou [2 ]
Kawai, Kenji [1 ]
机构
[1] Kochi Univ, Dept Aquaculture, Fish Dis Lab, Kochi 7838502, Japan
[2] Kochi Univ, Grad Sch Kuroshio Sci, Kochi 7838502, Japan
来源
FISH PATHOLOGY | 2011年 / 46卷 / 02期
关键词
bacterial adherence; gill; infection route; in vivo analysis; Photobacterium damselae subsp piscicida; SEABREAM SPARUS-AURATA; FISH EPITHELIAL-CELLS; PASTEURELLA-PISCICIDA; AEROMONAS-HYDROPHILA; HELA-CELLS; INVASION; INTERNALIZATION; EDWARDSIELLA; REPLICATION; PENETRATION;
D O I
10.3147/jsfp.46.45
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Pseudotuberculosis caused by Photobacterium damselae subsp. piscicida is a serious problem in fish culture industry. The initial stage of host-pathogen interaction is primarily important to understand infectious disease. However, the mode of transmission and route of infection of the agent are not fully studied. In this study, changes in the distribution of the bacterium on and in yellowtail Serbia quinqueradiata body during infection process was investigated using immersion challenge. The result revealed that external regions such as the lower jaw, pectoral fin, skin and gills permitted bacterial adherence soon after immersion. Viable bacterial counts increased in the gills soon after infection, then they increased in the blood, spleen and kidney. Moreover, fish were exposed to bacteria at the gills or posterior part of body surface to elucidate the primary infection route. The fish group challenged at gills showed higher mortality than the other group. These results reveal that the gills are the primary regions for P damselae subsp. piscicida to invade and proliferate in yellowtail.
引用
收藏
页码:45 / 50
页数:6
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