Protective effects of thymoquinone against acrylamide-induced liver, kidney and brain oxidative damage in rats

被引:81
作者
Abdel-Daim, Mohamed M. [1 ,2 ]
El-Ela, Fatma I. Abo [3 ]
Alshahrani, Fatima K. [4 ]
Bin-Jumah, May [5 ]
Al-Zharani, Mohammed [6 ]
Almutairi, Bader [1 ]
Alyousif, Mohamed S. [1 ]
Bungau, Simona [7 ]
Aleya, Lotfi [8 ]
Alkahtani, Saad [1 ]
机构
[1] King Saud Univ, Coll Sci, Dept Zool, POB 2455, Riyadh 11451, Saudi Arabia
[2] Suez Canal Univ, Fac Vet Med, Pharmacol Dept, Ismailia 41522, Egypt
[3] Beni Suef Univ, Fac Vet Med, Pharmacol Dept, Bani Suwayf, Egypt
[4] King Saud Univ, Coll Food & Agr Sci, Dept Food Sci & Nutr, Riyadh 11451, Saudi Arabia
[5] Princess Nourah Bint Abdulrahman Univ, Coll Sci, Biol Dept, Riyadh, Saudi Arabia
[6] Imam Mohammad Ibn Saud Islamic Univ IMSIU, Coll Sci, Biol Dept, Riyadh, Saudi Arabia
[7] Univ Oradea, Dept Pharm, Fac Med & Pharm, Oradea, Romania
[8] Bourgogne Franche Comte Univ, UMR CNRS 6249, Chronoenvironm Lab, F-25030 Besancon, France
关键词
Acrylamide; Thymoquinone; Antioxidant; Hepatotoxicity; Nephrotoxicity; Neurotoxicity; NIGELLA-SATIVA; NITRIC-OXIDE; STRESS; ANTIOXIDANT; TOXICITY; SUPEROXIDE; EXPRESSION; EXPOSURE; NEURONS; LEVEL;
D O I
10.1007/s11356-020-09516-3
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Acrylamide (AA), an industrial monomer, may cause multi-organ toxicity through induction of oxidative stress and inflammation. The antioxidant properties of thymoquinone (TQ), an active constituent ofNigella sativa, have been established before. The aim of the current study was to assess the protective effects of TQ against AA-induced toxicity in rats. Forty-eight male Wistar rats were divided into six groups each of eight rats. The first group acted as a negative control and received normal saline. Groups II and III were administered TQ orally at doses of 10 and 20 mg/kg b.wt., respectively, for 21 days. The four group received AA (20 mg/kg b.wt.) for 14 days. The five and six groups were given TQ at either dose for 21 days, starting seven days before AA supplementation (for 14 days). Acrylamide intoxication was associated with significant (p< 0.05) increases in serum levels of liver injury biomarkers (alanine transferase, aspartate transferase, and alkaline phosphatase), renal function products (urea, creatinine), DNA oxidative damage biomarker (8-oxo-2 '-deoxyguanosine), and pro-inflammatory biomarkers (interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha). Moreover, AA intoxication was associated with increased lipid peroxidation and nitric oxide levels, while reduced glutathione concentration and activities of glutathione peroxidase, superoxide dismutase, and catalase in the liver, kidney, and brain. TQ administration normalized AA-induced changes in most serum parameters and enhanced the antioxidant capacity in the liver, kidney, and brain tissues in a dose-dependent manner. In conclusion, the current experiment showed that TQ exerted protective and antioxidant activities against AA-induced toxicity in mice.
引用
收藏
页码:37709 / 37717
页数:9
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