Fluorescent high-performance liquid chromatography assay for lipophilic alcohols

被引：5

作者：

Nelson, Thomas J. ^{[1
]}

机构：

[1] Blanchette Rockefeller Neurosci Inst, Morgantown, WV 26505 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2011年 / 419卷 / 01期

关键词：

Alcohols; Bryostatin; Cholesterol; HPLC; Bodipy FL; Derivatization; DERIVATIZATION REAGENT;

D O I：

10.1016/j.ab.2011.07.036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new ultrasensitive fluorescent derivatization procedure for chromatographic analysis of primary, secondary, and nonpolar tertiary alcohols is described. The procedure uses Bodipy FL in basic dichloromethane solution with Mukaiyama's reagent (2-chloro-1-methylpyridinium iodide) to form highly fluorescent ester derivatives that can be separated by silica normal-phase high-performance liquid chromatography (HPLC). Rhodamine WT and Oregon green 488 were also useful derivatization reagents. The detection limit for detection of cholesterol and bryostatin by Bodipy FL was less than 1 fmol. The reaction conditions are gentle enough that low concentrations of unstable alcohols such as bryostatin 1 can be measured. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：40 / 45

页数：6

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