Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy

被引:22
作者
Smith-Moritz, Andreia M. [1 ,2 ]
Chern, Mawsheng [1 ,3 ]
Lao, Jeemeng [1 ,3 ]
Sze-To, Wing Hoi [1 ,3 ]
Heazlewood, Joshua L. [1 ,2 ]
Ronald, Pamela C. [1 ,2 ,3 ]
Vega-Sanchez, Miguel E. [1 ,3 ]
机构
[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Joint BioEnergy Inst, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA
[3] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
关键词
near infrared spectroscopy; cell wall; hemicellulose; multivariate analysis; mutant screen; pls modeling; FT-IR; GLUCURONOXYLAN BIOSYNTHESIS; CHEMICAL-COMPOSITION; ARABIDOPSIS; MUTANTS; CELLULOSE; IDENTIFICATION; SPECTRA; WOOD; MICROSPECTROSCOPY;
D O I
10.1186/1746-4811-7-26
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to construct a monosaccharide range predictive model using PLS regression. This model was then used to identify specific monosaccharide outliers from the mutant population.
引用
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页数:13
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