Fast haptoglobin phenotyping based on microchip electrophoresis

被引：11

作者：

Huang, Bingrong ^{[1
]}

Huang, Changgang ^{[1
,2
]}

Liu, Pingping ^{[1
]}

Wang, Fangfang ^{[1
]}

Na, Na ^{[1
]}

Ouyang, Jin ^{[1
]}

机构：

[1] Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China

[2] Wuhan Ctr Dis Prevent & Control, Wuhan 430015, Peoples R China

来源：

TALANTA | 2011年 / 85卷 / 01期

关键词：

Haptoglobin phenotype; Microchip electrophoresis; Laser-induced fluorescence detection; HEMOGLOBIN BINDING-CAPACITY; C-REACTIVE PROTEIN; CAPILLARY-ELECTROPHORESIS; POLYACRYLAMIDE-GELS; MICROFLUIDIC CHIP; SERUM HAPTOGLOBIN; POLYMORPHISM; SEPARATION; SYSTEM; RISK;

D O I：

10.1016/j.talanta.2011.03.066

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new and fast method for haptoglobin phenotyping was developed based on microchip electrophoresis with laser induced fluorescence detection. Haptoglobin phenotypes 1-1 and 2-2 were labeled with fluorescein isothiocyanate. The analyses were performed on glass microchip which was simply treated with sodium dodecyl sulfate. After the optimization of the separation conditions. Hp 1-1 and Hp 2-2 could be differentiated in 150s and the detection limits for Hp 1-1 and Hp 2-2 were 0.39 and 0.62 mu g/mL, respectively. Finally, the method was applied to human serum samples from healthy people and liver cancer patients. A decrease in Hp concentration for liver cancer patients was confirmed. Featuring high efficiency, speed, simplicity, the method reveals great potentials for the diagnosis of diseases and proteome research. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：333 / 338

页数：6

共 38 条

[1] HAPTOGLOBIN SUBTYPING BY ISOELECTRIC-FOCUSING IN MINIATURIZED POLYACRYLAMIDE GELS REHYDRATED IN PRESENCE OF 2-MERCAPTOETHANOL [J].

ALONSO, A ;

VISEDO, G ;

SANCHO, M ;

FERNANDEZPIQUERAS, J .

ELECTROPHORESIS, 1990, 11 (04) :321-324

[2] Study of serum haptoglobin and its glycoforms in the diagnosis of hepatocellular carcinoma: A glycoproteomic approach [J].

Ang, Irene L. ;

Poon, Terence C. W. ;

Lai, Paul B. S. ;

Chan, Anthony T. C. ;

Ngai, Sai-Ming ;

Hui, Alex Y. ;

Johnson, Philip J. ;

Sung, Joseph J. Y. .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (10) :2691-2700

[3]

Belder D, 2002, ELECTROPHORESIS, V23, P3567, DOI 10.1002/1522-2683(200210)23:20<3567::AID-ELPS3567>3.0.CO

[4]

2-3

[5] Detection of nitrated benzodiazepines by indirect laser-induced fluorescence detection on a microfluidic device [J].

Bishop, Sandra C. ;

Lerch, Margaret ;

McCord, Bruce R. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1154 (1-2) :481-484

[6] PROBLEMS IN QUANTITATIVE ESTIMATION OF HUMAN SERUM HAPTOGLOBIN BY SINGLE RADICAL IMMUNODIFFUSION [J].

BRAUN, HJ ;

ALY, FW .

CLINICA CHIMICA ACTA, 1969, 26 (03) :588-&

[7] Application of microchip assay system for the measurement of C-reactive protein in human saliva [J].

Christodoulides, N ;

Mohanty, S ;

Miller, CS ;

Langub, MC ;

Floriano, PN ;

Dharshan, P ;

Ali, MF ;

Bernard, B ;

Romanovicz, D ;

Anslyn, E ;

Fox, PC ;

McDevitt, JT .

LAB ON A CHIP, 2005, 5 (03) :261-269

[8] Fast determination of haptoglobin phenotype and calculation of hemoglobin binding capacity using high pressure gel permeation chromatography [J].

Delanghe, J ;

Allcock, K ;

Langlois, M ;

Claeys, L ;

De Buyzere, M .

CLINICA CHIMICA ACTA, 2000, 291 (01) :43-51

[9] Haptoglobin polymorphism and peripheral arterial occlusive disease [J].

Delanghe, J ;

Langlois, M ;

Duprez, D ;

De Buyzere, M ;

Clement, D .

ATHEROSCLEROSIS, 1999, 145 (02) :287-292

[10]

Delanghe J R, 1995, J Cardiovasc Risk, V2, P131, DOI 10.1097/00043798-199504000-00008

← 1 2 3 4 →