Comparison of polymerase chain reaction on fresh tissue samples and fecal drops on filter paper for detection of Trypanosoma cruzi in Rhodnius prolixus

被引:14
作者
Dorn, PL
Flores, J
Brahney, B
Gutierrez, A
Rosales, R
Rodas, A
Monroy, C
机构
[1] Loyola Univ, Dept Biol Sci, New Orleans, LA 70118 USA
[2] Univ San Carlos, Escuela Biol, Guatemala City, Guatemala
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2001年 / 96卷 / 04期
关键词
Trypanosoma cruzi; Rhodnius prolixus; PCR;
D O I
10.1590/S0074-02762001000400010
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
PCR detection of Trypanosoma cruzi in Rhodnius prolixus using fresh tissue or fecal drops on filter paper showed comparable results: 38.7% infection rate using the fresh tissue sample and 37.9% by dried fecal drop.
引用
收藏
页码:503 / 505
页数:3
相关论文
共 5 条
[1]   FIELD APPLICATION OF POLYMERASE CHAIN-REACTION DIAGNOSIS AND STRAIN TYPING OF TRYPANOSOMA-CRUZI IN BOLIVIAN TRIATOMINES [J].
BRENIERE, SF ;
BOSSENO, MF ;
TELLERIA, J ;
CARRASCO, R ;
VARGAS, F ;
YAKSIC, N ;
NOIREAU, F .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1995, 53 (02) :179-184
[2]   A SIMPLE PROTOCOL FOR THE PHYSICAL CLEAVAGE OF TRYPANOSOMA-CRUZI KINETOPLAST DNA PRESENT IN BLOOD-SAMPLES AND ITS USE IN POLYMERASE CHAIN-REACTION (PCR)-BASED DIAGNOSIS OF CHRONIC CHAGAS-DISEASE [J].
BRITTO, C ;
CARDOSO, MA ;
WINCKER, P ;
MOREL, CM .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1993, 88 (01) :171-172
[3]  
D'Alessandro-Bacigalupo Antonio, 1992, P1
[4]   Utility of the polymerase chain reaction in detection of Trypanosoma cruzi in Guatemalan Chagas' disease vectors [J].
Dorn, PL ;
Engelke, D ;
Rodas, A ;
Rosales, R ;
Melgar, S ;
Brahney, B ;
Flores, J ;
Monroy, C .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1999, 60 (05) :740-745
[5]   Simplified method for preservation and polymerase chain reaction amplification of Trypanosoma cruzi DNA in human blood [J].
Dorn, PL ;
Selgean, S ;
Guillot, M .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1997, 92 (02) :253-255