Measurement of gp130 cytokines - Oncostatin M and IL-6 in gingival crevicular fluid of patients with chronic periodontitis

被引:67
|
作者
Lin, SJ
Chen, YL
Kuo, MYB
Li, CL
Lu, HK [1 ]
机构
[1] Taipei Med Univ, Periodontal Clin Dent Dept, Taipei, Taiwan
[2] Shin Kong Wu Ho Su Mem Hosp, Taipei, Taiwan
[3] Natl Taiwan Univ, Sch Dent, Coll Med, Taipei, Taiwan
[4] Taipei Med Univ, Coll Oral Med, Periodontal Dept, Taipei 110, Taiwan
关键词
gingival crevicular fluid; interleukin-6; oncostatin M; periodontitis;
D O I
10.1016/j.cyto.2004.12.018
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several proinflammatory cytokines can induce periodontal tissue destruction and are thought to be useful indicators or diagnostic markers for periodontitis. Here, we aimed to investigate whether oncostatin M (OSM) was present in gingival crevicular fluid (GCF) and to clarify the correlation of GCF OSM and interleukin-6 (IL-6) levels with the severity of periodontitis. Sixty-two sites in 14 patients were divided into 4 groups based on probing depth (PD) and bleeding on probing (BOP). GCF was collected using paper strips from clinically health sites (PD <= 3 mm, CAL: 1-3 mm, without BOP, n = 3 1), mildly diseased sites (PD :! 3 mm, CAL: 3-5 mm, with BOP, n = 11), moderately diseased sites (PD = 4-6 mm, CAL: 5-8 mm, with BOP, it = 11), and severely diseased sites (PD > 6 mm, CAL: 8-12 mm, with BOP, n = 9). IL-6 and OSM in GCF were quantified by enzyme-linked immunosorbent assay and are expressed as concentrations (pg/ml) and total amounts (pg/site). Correlations of OSM and IL-6 levels with the severity of periodontitis in all groups were determined using Spearman rank correlation (r(s)). Our results showed that OSM and IL-6 were detected in most GCF samples. The total amounts of OSM and IL-6 were significantly positive correlated with severity of diseased sites (OSM: r(s) = 0.526, p < 0.01; IL-6: r(s) = 0.729, p < 0.01). No correlations of OSM or 11-6 concentration in GCF were found with disease severity. OSM and JL-6 levels in GCF were positively correlated to each other when expressed as either concentrations or total amounts (concentrations: r = 0.485, p < 0.01, total amounts r = 0.490, p < 0.01). In conclusion, our findings suggest that IL-6 and OSM may play a role in modulating the inflammatory cascade of chronic periodontitis. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:160 / 167
页数:8
相关论文
共 50 条
  • [31] Targeted disruption of the IL-6 related genes: gp130 and NF-IL-6
    Akira, S
    Yoshida, K
    Tanaka, T
    Taga, T
    Kishimoto, T
    IMMUNOLOGICAL REVIEWS, 1995, 148 : 221 - 253
  • [32] ONCOSTATIN-M BINDS DIRECTLY TO GP130 AND BEHAVES AS INTERLEUKIN-6 ANTAGONIST ON A CELL-LINE EXPRESSING GP130 BUT LACKING FUNCTIONAL ONCOSTATIN-M RECEPTORS
    SPORENO, E
    PAONESSA, G
    SALVATI, AL
    GRAZIANI, R
    DELMASTRO, P
    CILIBERTO, G
    TONIATTI, C
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (15) : 10991 - 10995
  • [33] T cell hyperreactivity to IL-6 in chronic nonviremic HBV carriers despite normal IL-6 receptor or gp130 expression
    Baroja, ML
    Sirit, FL
    Rivera, H
    Garzaro, J
    Bianco, NE
    Machado, IV
    VIRAL IMMUNOLOGY, 1996, 9 (03) : 187 - 194
  • [34] GINGIVAL CREVICULAR FLUID GLYCOSAMINOGLYCAN LEVELS IN PATIENTS WITH CHRONIC ADULT PERIODONTITIS
    SMITH, AJ
    ADDY, M
    EMBERY, G
    JOURNAL OF CLINICAL PERIODONTOLOGY, 1995, 22 (05) : 355 - 361
  • [35] The role of gp130/IL-6 cytokines in the development of pulmonary fibrosis: critical determinants of disease susceptibility and progression?
    Knight, DA
    Ernst, M
    Anderson, GP
    Moodley, YP
    Mutsaers, SE
    PHARMACOLOGY & THERAPEUTICS, 2003, 99 (03) : 327 - 338
  • [36] INVOLVEMENT OF GP130/IL-6 TRANSDUCING PROTEIN IN IL-11 RECEPTOR
    GASCAN, H
    FOURCIN, M
    LEBRUN, JJ
    WIDJENES, J
    POUPLARD, A
    CHEVALIER, S
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 19 - 19
  • [37] Signaling conformations of the tall cytokine receptor gp130 when in complex with IL-6 and IL-6 receptor
    Skiniotis, G
    Boulanger, MJ
    Garcia, KC
    Walz, T
    NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (06) : 545 - 551
  • [38] Signaling conformations of the tall cytokine receptor gp130 when in complex with IL-6 and IL-6 receptor
    Georgios Skiniotis
    Martin J Boulanger
    K Christopher Garcia
    Thomas Walz
    Nature Structural & Molecular Biology, 2005, 12 : 545 - 551
  • [39] THE MEMBRANE DISTAL HALF OF GP130 IS RESPONSIBLE FOR THE FORMATION OF A TERNARY COMPLEX WITH IL-6 AND THE IL-6 RECEPTOR
    HORSTEN, U
    SCHMITZVANDELEUR, H
    MULLBERG, J
    HEINRICH, PC
    ROSEJOHN, S
    FEBS LETTERS, 1995, 360 (01) : 43 - 46
  • [40] Interleukine-6 (IL-6) synthesis and gp130 expression by human pericardium
    Menet, E
    Corbi, P
    Ancey, C
    Morel, F
    Delwail, A
    Garcia, M
    Osta, AM
    Wijdenes, J
    Potreau, D
    Lecron, JC
    EUROPEAN CYTOKINE NETWORK, 2001, 12 (04) : 639 - 646