Measurement of gp130 cytokines - Oncostatin M and IL-6 in gingival crevicular fluid of patients with chronic periodontitis

被引:67
|
作者
Lin, SJ
Chen, YL
Kuo, MYB
Li, CL
Lu, HK [1 ]
机构
[1] Taipei Med Univ, Periodontal Clin Dent Dept, Taipei, Taiwan
[2] Shin Kong Wu Ho Su Mem Hosp, Taipei, Taiwan
[3] Natl Taiwan Univ, Sch Dent, Coll Med, Taipei, Taiwan
[4] Taipei Med Univ, Coll Oral Med, Periodontal Dept, Taipei 110, Taiwan
关键词
gingival crevicular fluid; interleukin-6; oncostatin M; periodontitis;
D O I
10.1016/j.cyto.2004.12.018
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several proinflammatory cytokines can induce periodontal tissue destruction and are thought to be useful indicators or diagnostic markers for periodontitis. Here, we aimed to investigate whether oncostatin M (OSM) was present in gingival crevicular fluid (GCF) and to clarify the correlation of GCF OSM and interleukin-6 (IL-6) levels with the severity of periodontitis. Sixty-two sites in 14 patients were divided into 4 groups based on probing depth (PD) and bleeding on probing (BOP). GCF was collected using paper strips from clinically health sites (PD <= 3 mm, CAL: 1-3 mm, without BOP, n = 3 1), mildly diseased sites (PD :! 3 mm, CAL: 3-5 mm, with BOP, n = 11), moderately diseased sites (PD = 4-6 mm, CAL: 5-8 mm, with BOP, it = 11), and severely diseased sites (PD > 6 mm, CAL: 8-12 mm, with BOP, n = 9). IL-6 and OSM in GCF were quantified by enzyme-linked immunosorbent assay and are expressed as concentrations (pg/ml) and total amounts (pg/site). Correlations of OSM and IL-6 levels with the severity of periodontitis in all groups were determined using Spearman rank correlation (r(s)). Our results showed that OSM and IL-6 were detected in most GCF samples. The total amounts of OSM and IL-6 were significantly positive correlated with severity of diseased sites (OSM: r(s) = 0.526, p < 0.01; IL-6: r(s) = 0.729, p < 0.01). No correlations of OSM or 11-6 concentration in GCF were found with disease severity. OSM and JL-6 levels in GCF were positively correlated to each other when expressed as either concentrations or total amounts (concentrations: r = 0.485, p < 0.01, total amounts r = 0.490, p < 0.01). In conclusion, our findings suggest that IL-6 and OSM may play a role in modulating the inflammatory cascade of chronic periodontitis. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:160 / 167
页数:8
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