Seonamhaeicola sediminis sp. nov., isolated from marine sediment

A novel Gram-stain-negative, orange, rod or curved rod, facultatively anaerobic, gliding bacterial strain, designated strain W255(T), was isolated from Xiaoshi Island, Weihai, China. The strain W255(T) grows optimally at a 28 degrees C, pH 7.5, and 3.0% (w/v) NaCl environment. Its colonies are circular, orange, non-transparent, smooth, and approximately 0.2-0.8 mm in diameter, after being cultured for 72 h on marine agar 2216. Cells of the strain W255(T) are 0.3-0.8 mu m wide and 1.0-4.0 mu m long, catalase-positive and oxidase-positive. The major cellular fatty acids are iso-C-15:0, iso-C-15:1 G, and iso-C-15:0 3-OH. The sole respiratory quinone is MK-6. The major polar lipids include phosphatidylethanolamine, one unidentified amino lipid, one amino glycolipid, and two unidentified lipids (L1 and L2). The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W255(T) has the highest similarities with the type strain of the type species of the genus Seonamhaeicola, S. aphaedonensis KCTC 32578(T) (97.2%), and moderate with 'S. acroporae' KCTC 62713(T) (96.5%), S. algicola Gy8(T) (95.4%) and S. marinus B011(T) (94.5%). The ANI and dDDH values between strain W255(T) and S. aphaedonensis KCTC 32578(T) are 86.6% and 31.3%, respectively. The genomic DNA G + C content is 33.5 mol%. On the basis of gene annotation, it was observed that strain W255(T) have the abilities of nitrate reduction and utilizing various carbon sources, suggesting that this strain might be an important participant in the nitrogen cycle and carbon cycle in the marine environment. Based on the phenotypic, chemotaxonomic and phylogenetic analysis, strain W255(T) has been considered as a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola sediminis sp. nov. is proposed. The type strain is W255(T) (= MCCC 1H00377(T) = KCTC 72085(T)).