Resveratrol reverses the adverse effects of bevacizumab on cultured ARPE-19 cells

被引：31
作者：
Subramani, Murali [1 ,5 ]
Ponnalagu, Murugeswari [1 ]
Krishna, Lekshmi [1 ]
Jeyabalan, Nallathambi [2 ]
Chevour, Priyanka [2 ]
Sharma, Anupam [2 ]
Jayadev, Chaitra [3 ]
Shetty, Rohit [4 ]
Begum, Nargis [5 ]
Archunan, Govindaraju [6 ]
Das, Debashish [1 ]
机构：
[1] Narayana Nethralaya Fdn, Stem Cell Lab, GROW Labs, Bangalore, Karnataka, India
[2] Narayana Nethralaya Fdn, GROW Labs, Bangalore, Karnataka, India
[3] Narayana Nethralaya Eye Hosp, Vitreoretina Serv, Bangalore, Karnataka, India
[4] Narayana Nethralaya Eye Hosp, Dept Cornea & Refract Surg, Bangalore, Karnataka, India
[5] Jamal Mohammed Coll, Postgrad Dept Biotechnol, Tiruchirappalli, Tamil Nadu, India
[6] Bharatidasan Univ, Dept Anim Sci, Tiruchirappalli, Tamil Nadu, India
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
PIGMENT EPITHELIAL-CELLS; DIABETIC MACULAR EDEMA; TO-MESENCHYMAL TRANSITION; GROWTH-FACTOR PHARMACOTHERAPY; ANTI-VEGF AGENTS; MEDIATED INHIBITION; OXIDATIVE STRESS; CROSS-LINKING; EXPRESSION; LIMBAL;
D O I：
10.1038/s41598-017-12496-z
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR) are one of the major causes of blindness caused by neo-vascular changes in the retina. Intravitreal anti-VEGF injections are widely used in the treatment of wet-AMD and PDR. A significant percentage of treated patients have complications of repeated injections. Resveratrol (RES) is a polyphenol phytoalexin with anti-oxidative, anti-inflammatory and anti-proliferative properties. Hence, we hypothesized that if RES is used in combination with bevacizumab (BEV, anti-VEGF), it could reverse the adverse effects that precipitate fibrotic changes, drusen formation, tractional retinal detachment and so on. Human retinal pigment epithelial cells were treated with various combinations of BEV and RES. There was partial reduction in secreted VEGF levels compared to untreated controls. Epithelial-mesenchymal transition was lower in BEV + RES treated cultures compared to BEV treated cultures. The proliferation status was similar in BEV + RES as well as BEV treated cultures both groups. Phagocytosis was enhanced in the presence of BEV + RES compared to BEV. Furthermore, we observed that notch signaling was involved in reversing the adverse effects of BEV. This study paves way for a combinatorial strategy to treat as well as prevent adverse effects of therapy in patients with wet AMD and PDR.
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页数：16
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