Trypanosoma evansi: Identification and characterization of a variant surface glycoprotein lacking cysteine residues in its C-terminal domain

被引:4
作者
Jia, Yonggen [1 ]
Zhao, Xinxin [1 ]
Zou, Jingru [1 ]
Suo, Xun [1 ]
机构
[1] China Agr Univ, Parasitol Lab, Coll Vet Med, Beijing 100193, Peoples R China
基金
中国国家自然科学基金;
关键词
Trypanosoma brucei; Trypanosoma evansi; Antigenic variation; Variant surface glycoprotein; C-terminal domain; Cysteine residues; ANTIGENIC VARIATION; AFRICAN TRYPANOSOMES; VSG GENE; BRUCEI; CONGOLENSE; EXPRESSION; TRANSPOSITION; GLYCOSYLATION; PARASITE; GENOME;
D O I
10.1016/j.exppara.2010.06.035
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
African trypanosomes are flagellated unicellular parasites which proliferate extracellularly in the mammalian host blood-stream and tissue spaces. They evade the hosts' antibody-mediated lyses by sequentially changing their variant surface glycoprotein (VSG). VSG tightly coats the entire parasite body, serving as a physical barrier. In Trypanosoma brucei and the closely related species Trypanosoma evansi, Trypanosoma equiperdum, each VSG polypeptide can be divided into N- and C-terminal domains, based on cysteine distribution and sequence homology. N-terminal domain, the basis of antigenic variation, is hypervariable and contains all the exposed epitopes; C-terminal domain is relatively conserved and a full set of four or eight cysteines were generally observed. We cloned two genes from two distinct variants of T. evansi, utilizing RT-PCR with VSG-specific primers. One contained a VSG type A N-terminal domain followed a C-terminal domain lacking cysteine residues. To confirm that this gene is expressed as a functional VSG, the expression and localization of the corresponding gene product were characterized using Western blotting and immunofluorescent staining of living trypanosomes. Expression analysis showed that this protein was highly expressed, variant-specific, and had a ubiquitous cellular surface localization. All these results indicated that it was expressed as a functional VSG. Our finding showed that cysteine residues in VSG C-terminal domain were not essential; the conserved C-terminal domain generally in T. brucei like VSGs would possibly evolve for regulating the VSG expression. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:100 / 106
页数:7
相关论文
共 40 条
  • [1] Barry JD, 2001, ADV PARASIT, V49, P1, DOI 10.1016/S0065-308X(01)49037-3
  • [2] ACTIVATION OF TRYPANOSOME SURFACE GLYCOPROTEIN GENES INVOLVES A DUPLICATION-TRANSPOSITION LEADING TO AN ALTERED 3' END
    BERNARDS, A
    VANDERPLOEG, LHT
    FRASCH, ACC
    BORST, P
    BOOTHROYD, JC
    COLEMAN, S
    CROSS, GAM
    [J]. CELL, 1981, 27 (03) : 497 - 505
  • [3] The genome of the African trypanosome Trypanosoma brucei
    Berriman, M
    Ghedin, E
    Hertz-Fowler, C
    Blandin, G
    Renauld, H
    Bartholomeu, DC
    Lennard, NJ
    Caler, E
    Hamlin, NE
    Haas, B
    Böhme, W
    Hannick, L
    Aslett, MA
    Shallom, J
    Marcello, L
    Hou, LH
    Wickstead, B
    Alsmark, UCM
    Arrowsmith, C
    Atkin, RJ
    Barron, AJ
    Bringaud, F
    Brooks, K
    Carrington, M
    Cherevach, I
    Chillingworth, TJ
    Churcher, C
    Clark, LN
    Corton, CH
    Cronin, A
    Davies, RM
    Doggett, J
    Djikeng, A
    Feldblyum, T
    Field, MC
    Fraser, A
    Goodhead, I
    Hance, Z
    Harper, D
    Harris, BR
    Hauser, H
    Hostetter, J
    Ivens, A
    Jagels, K
    Johnson, D
    Johnson, J
    Jones, K
    Kerhornou, AX
    Koo, H
    Larke, N
    [J]. SCIENCE, 2005, 309 (5733) : 416 - 422
  • [4] A STRUCTURAL MOTIF IN THE VARIANT SURFACE GLYCOPROTEINS OF TRYPANOSOMA-BRUCEI
    BLUM, ML
    DOWN, JA
    GURNETT, AM
    CARRINGTON, M
    TURNER, MJ
    WILEY, DC
    [J]. NATURE, 1993, 362 (6421) : 603 - 609
  • [5] A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma brucei
    Boothroyd, Catharine E.
    Dreesen, Oliver
    Leonova, Tatyana
    Ly, K. Ina
    Figueiredo, Luisa M.
    Cross, George A. M.
    Papavasiliou, F. Nina
    [J]. NATURE, 2009, 459 (7244) : 278 - U168
  • [6] Determination of the disulfide bonds within a B domain variant surface glycoprotein from Trypanosoma congolense
    Bussler, H
    Linder, M
    Linder, D
    Reinwald, E
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (49) : 32582 - 32586
  • [7] THE 5'-LIMIT OF TRANSPOSITION AND UPSTREAM BARREN REGION OF A TRYPANOSOME VSG GENE - TANDEM-76 BASE-PAIR REPEATS FLANKING (TAA)90
    CAMPBELL, DA
    VANBREE, MP
    BOOTHROYD, JC
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (06) : 2759 - 2774
  • [8] VARIANT SPECIFIC GLYCOPROTEIN OF TRYPANOSOMA-BRUCEI CONSISTS OF 2 DOMAINS EACH HAVING AN INDEPENDENTLY CONSERVED PATTERN OF CYSTEINE RESIDUES
    CARRINGTON, M
    MILLER, N
    BLUM, M
    RODITI, I
    WILEY, D
    TURNER, M
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (03) : 823 - 835
  • [9] Structure of the C-terminal domain from Trypanosoma brucei variant surface glycoprotein MITat1.2
    Chattopadhyay, A
    Jones, NG
    Nietlispach, D
    Nielsen, PR
    Voorheis, HP
    Mott, HR
    Carrington, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (08) : 7228 - 7235
  • [10] IDENTIFICATION, PURIFICATION AND PROPERTIES OF CLONE-SPECIFIC GLYCOPROTEIN ANTIGENS CONSTITUTING SURFACE COAT OF TRYPANOSOMA-BRUCEI
    CROSS, GAM
    [J]. PARASITOLOGY, 1975, 71 (DEC) : 393 - +