Human neural progenitors from different foetal forebrain regions remyelinate the adult mouse spinal cord

被引:35
作者
Buchet, Delphine
Garcia, Corina
Deboux, Cyrille
Nait-Oumesmar, Brahim [2 ]
Baron-Van Evercooren, Anne [1 ,2 ]
机构
[1] Univ Paris 06, Ctr Rech, Inst Cerveau & Moelle Epiniere, Inserm,CNRS,UMR S 975,U975,UMR 7225, F-75651 Paris 13, France
[2] Hop La Pitie Salpetriere, AP HP, Federat Neurol, Paris, France
关键词
human neural progenitor cells; oligodendrocyte; migration; oligodendrogenesis; remyelination; STEM-CELL TRANSPLANTATION; SUBCORTICAL WHITE-MATTER; IN-VITRO; PRECURSOR CELLS; OLIGODENDROCYTE DEVELOPMENT; MULTIPLE-SCLEROSIS; SHIVERER MOUSE; MYELIN REPAIR; HUMAN BRAIN; RAT-BRAIN;
D O I
10.1093/brain/awr030
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Improving oligodendroglial differentiation from human foetal neural progenitor cells remains a primordial issue to accomplish successful cell-based therapies in myelin diseases. Here, we combined in situ, in vitro and in vivo approaches to assess the oligodendrogenic potential of different human foetal forebrain regions during the first trimester of gestation. We show for the first time that the initial wave of oligodendrocyte progenitor emergence in the ventral telencephalon onsets as early as 7.5 weeks into gestation. Interestingly, in vitro, isolation of ganglionic eminences yielded oligodendrocyte progenitor-enriched cultures, as compared with cortex and thalamus. Most importantly, single injection of human neural progenitors into rodent models of focal gliotoxic demyelination revealed the great capacity of these cells to survive, extensively migrate and successfully remyelinate the spinal cord, irrespective of their origin. Thus, our study brings novel insights into the biology of early human foetal neural progenitor cells and offers new support for the development of cellular therapeutics for myelin disorders.
引用
收藏
页码:1168 / 1183
页数:16
相关论文
共 57 条
[1]  
Back SA, 2001, J NEUROSCI, V21, P1302
[2]   Chimeric brains generated by intraventricular transplantation of fetal human brain cells into embryonic rats [J].
Brüstle, O ;
Choudhary, K ;
Karram, K ;
Hüttner, A ;
Murray, K ;
Dubois-Dalcq, M ;
McKay, RDG .
NATURE BIOTECHNOLOGY, 1998, 16 (11) :1040-1044
[3]   NEUROEPITHELIAL PROGENITOR CELLS EXPLANTED FROM HUMAN FETAL BRAIN PROLIFERATE AND DIFFERENTIATE IN-VITRO [J].
BUCCARON, MH .
NEUROBIOLOGY OF DISEASE, 1995, 2 (01) :37-47
[4]   Long-term fate of human telencephalic progenitor cells grafted into the adult mouse brain:: Effects of previous amplification in vitro [J].
Buchet, D ;
Buc-Caron, MH ;
Sabaté, O ;
Lachapelle, F ;
Mallet, J .
JOURNAL OF NEUROSCIENCE RESEARCH, 2002, 68 (03) :276-283
[5]   Long-term expression of β-glucuronidase by genetically modified human neural progenitor cells grafted into the mouse central nervous system [J].
Buchet, D ;
Serguera, C ;
Zennou, W ;
Charneau, P ;
Mallet, J .
MOLECULAR AND CELLULAR NEUROSCIENCE, 2002, 19 (03) :389-401
[6]   In search of human oligodendroglia for myelin repair [J].
Buchet, Delphine ;
Baron-Van Evercooren, Anne .
NEUROSCIENCE LETTERS, 2009, 456 (03) :112-119
[7]   In vitro expansion of a multipotent population of human neural progenitor cells [J].
Carpenter, MK ;
Cui, X ;
Hu, ZY ;
Jackson, J ;
Sherman, S ;
Seiger, Å ;
Wahlberg, LU .
EXPERIMENTAL NEUROLOGY, 1999, 158 (02) :265-278
[8]   Differential generation of oligodendrocytes from human and rodent embryonic spinal cord neural precursors [J].
Chandran, S ;
Compston, A ;
Jauniaux, E ;
Gilson, J ;
Blakemore, W ;
Svendsen, C .
GLIA, 2004, 47 (04) :314-324
[9]   Introduction: stem cells and brain repair [J].
Chandran, Siddharthan ;
Caldwell, Maeve ;
Allen, Nick .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2008, 363 (1489) :5-7
[10]   Late Origin of Glia-Restricted Progenitors in the Developing Mouse Cerebral Cortex [J].
Costa, Marcos R. ;
Bucholz, Oliver ;
Schroeder, Timm ;
Goetz, Magdalena .
CEREBRAL CORTEX, 2009, 19 :I135-I143