Extraction of chlorophyll a from biological soil crusts: A comparison of solvents for spectrophotometric determination

被引：133

作者：

Castle, Sarah C. ^{[1
]}

Morrison, Conor D. ^{[1
]}

Barger, Nichole N. ^{[1
]}

机构：

[1] Univ Colorado, Boulder, CO 80309 USA

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2011年 / 43卷 / 04期

关键词：

Biological soil crust; Chlorophyll a; Dimethyl sulfoxide; DMSO; Ethanol; Methanol; Acetone; ETHANOL SOLVENTS; EQUATIONS; METHANOL; ACETONE; DESERT;

D O I：

10.1016/j.soilbio.2010.11.025

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

We tested the efficacy of four different commonly used solvents (acetone, ethanol, dimethyl sulfoxide, methanol) for the extraction of chla from biological soil crusts of three different successional stages (dark, intermediate, and light). Our results indicate that a double extraction technique is necessary in order to achieve chla recovery in the range of 76-87 percent. For all crust types, ethanol and dimethyl sulfoxide extracted the greatest amount of chla using a two-extraction efficiency calculation. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：853 / 856

页数：4

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