Antibacterial effects of amino acids-grafted water-soluble chitosan against drug-resistant bacteria

被引：8

作者：

Kim, Jun-Ho ^{[1
]}

Kim, Nam-Hong ^{[1
]}

Kim, Eun-Ji ^{[1
]}

Kim, Ji Ho ^{[1
]}

Lee, Min-Young ^{[2
]}

Park, Yung-Hoon ^{[1
]}

Lee, Jung Ro ^{[3
]}

Park, Seong-Cheol ^{[1
]}

Jang, Mi-Kyeong ^{[1
]}

机构：

[1] Sunchon Natl Univ, Coll Engn, Dept Polymer Sci & Engn, Sunchon 540950, South Korea

[2] Gyeongsang Natl Univ, Sch Med, Dept Microbiol, Jinju 660750, South Korea

[3] Natl Inst Ecol, Seocheon 325813, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2016年 / 21卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

water-soluble chitosan; drug-resistant bacteria; antibacterial activity; amino acid-graft; Pseudomonas aeruginosa; ANTIMICROBIAL PEPTIDE; MULTIDRUG-RESISTANT; INFECTIONS; MECHANISM;

D O I：

10.1007/s12257-016-0144-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chitosan is an available natural polysaccharide that is biocompatible, biodegradable, and non-toxic. In this study, to enhance the antibacterial activity of water-soluble chitosan (WSC), amino acids with hydrophobic, anionic, or cationic side chains were grafted to the amine groups of WSC (amino acid-grafted WSC; AGW). Chemical characterization of AGW was performed by H-1 nuclear magnetic resonance spectroscopy, resulting in grafting molecular percentages of 7.3 similar to 9.9%. AGW exhibited more potent antibacterial activity than unmodified WSC in both drug-susceptible and drug-resistant bacteria. Scanning electron microscopy and a calcein leakage assay in bacteria and artificial liposomes, respectively, revealed the antibacterial action of AGW to be membranolytic. Lysine or isoleucinegrafted WSC showed potent in vivo antibacterial activity in a drug-resistant Pseudomonas aeruginosa-infected mouse model. AGW has potential use as an effective antibacterial agent.

引用

页码：183 / 189

页数：7

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