Improvements in the CRISPR/Cas9 system for high efficiency gene disruption in Trypanosoma cruzi

被引：19

作者：

Romagnoli, Bruno A. A. ^{[1
]}

Picchi, Gisele F. A. ^{[2
]}

Hiraiwa, Priscila M. ^{[3
]}

Borges, Beatriz S. ^{[4
]}

Alves, Lysangela R. ^{[1
]}

Goldenberg, Samuel ^{[1
]}

机构：

[1] Inst Carlos Chagas FIOCRUZ Parana, Lab Regulacao Expressao Gen, BR-81350010 Curitiba, Parana, Brazil

[2] Inst Carlos Chagas FIOCRUZ Parana, Lab Biol Mol Tripanossomatideos, BR-81350010 Curitiba, Parana, Brazil

[3] Inst Carlos Chagas FIOCRUZ Parand, Flow Cytometry Facil, BR-81350010 Curitiba, Parana, Brazil

[4] Inst Carlos Chagas FIOCRUZ Parana, Microscopy Facil, BR-81350010 Curitiba, Parana, Brazil

来源：

ACTA TROPICA | 2018年 / 178卷

关键词：

Trypanosoma cruzi; Knockout; CRISPR/Cas9; Improved technology; Essential genes; EXPRESSION; DELETION;

D O I：

10.1016/j.actatropica.2017.11.013

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects millions of individuals around the world. Although it has been known for more than a century, the study of T. cruzi has been a challenge, particularly due to the scarcity of tools for genome inquiries. Recently, strategies have been described allowing gene disruption in T. cruzi by the CRISPR/Cas9 nuclease system. Although these strategies demonstrated success in deleting some genes, several aspects could be improved to increase the efficiency of the CRISPR/Cas9 system in T. cruzi. Here, we report a strategy, based on adaptations and improvements of the two previously described systems, that results in efficient gene disruption that can be applied to any target, including the study of essential genes.

引用

页码：190 / 195

页数：6

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