Comparison of conventional susceptibility testing, penicillin-binding protein 2a latex agglutination testing, and mecA real-time PCR for detection of oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus

被引:9
作者
Miller, MB
Meyer, H
Rogers, E
Gilligan, PH
机构
[1] Univ N Carolina, Sch Med, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Sch Med, Dept Microbiol, Chapel Hill, NC 27599 USA
[3] Univ N Carolina Hosp, Chapel Hill, NC USA
关键词
D O I
10.1128/JCM.43.7.3450-3452.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Penicillin-binding protein (PBP) 2a latex agglutination was compared with conventional susceptibility testing and mecA real-time PCR for the detection of oxacillin resistance in Staphylococcus aureus. Inoculum volume and induction with oxacillin were PBP 2a testing variables. For coagulase-negative Staphylococcus, an increased inoculum volume of 10 mu l greatly reduced the number of isolates requiring induction.
引用
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页码:3450 / 3452
页数:3
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