Oxygen metabolism in plant/bacteria interactions: Effect of DPI on the pseudo-NAD(P)H oxidase activity of peroxidase

被引：23
作者：
Baker, CJ
Deahl, K
Domek, J
Orlandi, EW
机构：
[1] USDA ARS, Microbiol & Plant Pathol Lab, Beltsville, MD 20705 USA
[2] USDA ARS, Vegetable Lab, Beltsville, MD 20705 USA
[3] Univ Maryland, Dept Microbiol, College Pk, MD 20742 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 252卷 / 02期
关键词：
reactive oxygen; hydrogen peroxide; peroxidase; diphenyleneiodonium;
D O I：
10.1006/bbrc.1998.9665
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Diphenyleneiodonium (DPI) has been used frequently as a specific inhibitor of NADH oxidase activity in studies of plant/pathogen interactions. The present study reports the effect of DPI on the pseudo-oxidative activity of horseradish peroxidase, DPI, like other phenolics, is able to catalytically stimulate NADH oxidation in the presence of exogenous H2O2. The stimulated NADH oxidation has an acidic pH optimum and has an apparent Km of 111 mu M NADH. The NADH oxidation rate is linearly proportional to [DPI] and the amount of NADH oxidized is proportional to [H2O2]. Once exogenous H2O2 is depleted, the NADH oxidation is abruptly halted until additional H2O2 is supplied. In some respects DPI appears to mimic the effects of certain anti-oxidants that use HRP to scavenge H2O2 and NAD(P)H as a reductant, (C) 1998 Academic Press.
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收藏
页码：461 / 464
页数：4
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