Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease

被引：10

作者：

Balint, Adam ^{[1
]}

Balka, Gyula ^{[2
]}

Horvath, Peter ^{[3
]}

Kecskemeti, Sandor ^{[1
]}

Dan, Adam ^{[1
]}

Farsang, Attila ^{[4
]}

Szeredi, Levente ^{[1
]}

Banyai, Krisztian ^{[3
]}

Bartha, Daniel ^{[3
]}

Olasz, Ferenc ^{[3
]}

Belak, Sandor ^{[5
]}

Zadori, Zoltan ^{[3
]}

机构：

[1] Natl Food Chain Safety Off, Vet Diagnost Directorate, H-1149 Budapest, Hungary

[2] Szent Istvan Univ, Dept Pathol, Fac Vet Sci, H-1078 Budapest, Hungary

[3] Hungarian Acad Sci, Vet Med Res Inst, Agr Res Ctr, H-1143 Budapest, Hungary

[4] Natl Food Chain Safety Off, Directorate Vet Med Prod, H-1107 Budapest, Hungary

[5] SVA, Natl Vet Inst, Dept Virol Immunobiol & Parasitol, S-75189 Uppsala, Sweden

来源：

ARCHIVES OF VIROLOGY | 2015年 / 160卷 / 02期

关键词：

PRRSV; Sequencing; Complete genome; Epitope; AMINO-ACID-RESIDUES; MYCOPLASMA-HYOPNEUMONIAE; NONSTRUCTURAL PROTEIN-1; MONOCLONAL-ANTIBODY; CELL EPITOPES; IDENTIFICATION; NEUTRALIZATION; DISSECTION; DIVERSITY; EMERGENCE;

D O I：

10.1007/s00705-014-2265-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here, we report the isolation of a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory problems and high fever. Next-generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, and shows the highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 revealed no evidence of recombination but did show a high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of selective immune pressure in the evolution of PRRSV 9625/2012.

引用

页码：417 / 422

页数：6

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