Analysis of bovine pituitary specific transcription factor-Hinf I gene polymorphism in Indian zebuine cattle

被引：13

作者：

Mukesh, M. ^{[1
]}

Sodhi, M. ^{[1
]}

Sobti, R. C. ^{[2
]}

Prakash, B. ^{[1
]}

Kaushik, R. ^{[1
]}

Aggarwal, R. A. K. ^{[1
]}

Mishra, B. P. ^{[1
]}

机构：

[1] Natl Bur Anim Genet Resources, Cattle Genom Lab, Karnal 132001, Haryana, India

[2] Panjab Univ, Dept Biotechnol, Chandigarh 160014, India

来源：

LIVESTOCK SCIENCE | 2008年 / 113卷 / 01期

关键词：

PCR-RFLP; Pit-I gene; polymorphism; Indian zebu cattle;

D O I：

10.1016/j.livsci.2007.02.020

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The pituitary specific transcription factor (Pit-1) controls the expression of prolactin, thyrotropin, and growth hormone genes and is considered as an important candidate gene for several quantitative traits. The study was aimed to reveal the distribution pattern of known variants at Pit-1/HinfI locus in 16 distinct Indian native cattle (Bos indicus) breeds. PCR-RFLP analysis of 1350 bp fragment revealed the predominance of BB genotype and B allele with a mean frequency of 0.881 and 0.937, respectively, irrespective of utility type (dairy, dual, draft), geographical region (northern, central and southern) and coat colour (red/brown and grey/white). AB genotype was the next most frequent with a mean frequency of 0.119. Interestingly, only a single individual out of 723 screened was observed to have AA genotype. The significantly low frequency of A allele observed in present study differs from Bos taurine breeds. Chi square test showed non-significant (P > 0.05) difference in pattern of variant distribution, regardless of the geographical location, agroclimatic regions and coat colour. However, significant differences (P < 0.05) were observed between utility type categories (dual Vs draft and dairy vs draft). This study further validates the utility of PCR-RFLP as efficient means of exploring the distribution pattern of known variants at important candidate genes in Indian zebu cattle for future breeding plans. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：81 / 86

页数：6

共 26 条

[1]

Beauchemin VR, 2006, GENET MOL RES, V5, P438

[2] THE PITUITARY-SPECIFIC TRANSCRIPTION FACTOR-GHF-1 IS A HOMEOBOX-CONTAINING PROTEIN [J].

BODNER, M ;

CASTRILLO, JL ;

THEILL, LE ;

DEERINCK, T ;

ELLISMAN, M ;

KARIN, M .

CELL, 1988, 55 (03) :505-518

[3]

CARRIJO SM, 2003, P 49 C NAC GEN AG LI

[4] FUNCTION OF THE HOMEODOMAIN PROTEIN GHF1 IN PITUITARY CELL-PROLIFERATION [J].

CASTRILLO, JL ;

THEILL, LE ;

KARIN, M .

SCIENCE, 1991, 253 (5016) :197-199

[5] Association of bGH and Pit-1 gene variants with milk production traits in dairy Gyr bulls [J].

de Mattos, KK ;

Del Lama, SN ;

Martinez, ML ;

Freitas, AF .

PESQUISA AGROPECUARIA BRASILEIRA, 2004, 39 (02) :147-150

[6] Lack of association of GH1 and POU1F1 gene variants with meat production traits in Piemontese cattle [J].

Di Stasio, L ;

Sartore, S ;

Albera, A .

ANIMAL GENETICS, 2002, 33 (01) :61-64

[7] PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black-and-White cattle [J].

Dybus, A ;

Szatkowska, I ;

Czerniawska-Piatkowska, E ;

Grzesiak, W ;

Wójcik, J ;

Rzewucka, E ;

Zych, S .

ARCHIV FUR TIERZUCHT-ARCHIVES OF ANIMAL BREEDING, 2004, 47 (06) :557-563

[8] Associations between polymorphisms of growth hormone releasing hormone (GHRH) and pituitary transcription factor 1 (PIT1) genes and production traits of Limousine cattle [J].

Dybus, A ;

Kmiec, M ;

Sobek, Z ;

Pietrzyk, W ;

Wisniewski, B .

ARCHIV FUR TIERZUCHT-ARCHIVES OF ANIMAL BREEDING, 2003, 46 (06) :527-534

[9]

Hori-Oshima S, 2002, J ANIM SCI S1, V81, P252

[10]

Klauzinska Malgorzata, 2000, Animal Science Papers and Reports, V18, P107

← 1 2 3 →