Two mathematical models for the correction of carbohydrate and protein interference in the determination of uronic acids by the m-hydroxydiphenyl method

被引：26

作者：

Murado, MA ^{[1
]}

Vázquez, JA ^{[1
]}

Montemayor, MI ^{[1
]}

Cabo, ML ^{[1
]}

González, MD ^{[1
]}

机构：

[1] CSIC, Inst Invest Marinas, Vigo 36208, Galicia, Spain

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2005年 / 41卷

关键词：

glucose and protein interference; hyaluronic acid (HA); m-hydroxydiphenyl method; kinetic model; uronic acids;

D O I：

10.1042/BA20040127

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The most common method in the routine determination of uronic acids, the m-hydroxydiphenyl reaction, recently adapted to rapid microplate analysis, has as a main inconvenience, in any one of their modalities, interferences due to the frequent presence of proteins and neutral carbohydrates in the samples. Corresponding corrections in the literature are unsatisfactory when applied to complex matrices, and further adaptation to the microplate analysis is not free from additional problems. With particular reference to hyaluronic acid, the interactions between the principal reactants and the interfering materials are studied kinetically under realistic conditions, and simple mathematical models are proposed which satisfactorily describe the experimental results and allow adequate corrections to be made.

引用

页码：209 / 216

页数：8

共 12 条

[1]

Akhnazarova S., 1982, EXPT OPTIMIZATION CH

[2] NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].