Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes

被引：30

作者：

Al'Khafaji, Aziz M. ^{[1
,2
]}

Deatherage, Daniel ^{[2
]}

Brock, Amy ^{[1
,2
]}

机构：

[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[2] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA

来源：

ACS SYNTHETIC BIOLOGY | 2018年 / 7卷 / 10期

基金：

美国国家卫生研究院;

关键词：

lineage tracing; heterogeneity; barcode; clonal dynamics; population variation; CELL HETEROGENEITY; SINGLE CELLS; CANCER; EVOLUTION; DYNAMICS; IDENTIFICATION; TRACKING; MUTATION;

D O I：

10.1021/acssynbio.8b00105

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lineage tracking delivers essential quantitative insight into dynamic, probabilistic cellular processes, such as somatic tumor evolution and differentiation. Methods for high diversity lineage quantitation rely on sequencing a population of DNA barcodes. However, manipulation of specific individual lineages is not possible with this approach. To address this challenge, we developed a functionalized lineage tracing tool, Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT), that enables high diversity lineage tracing and lineage-specific manipulation of gene expression. This modular platform utilizes expressed barcode gRNAs to both track cell lineages and direct lineage-specific gene expression.

引用

页码：2468 / 2474

页数：13

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