Study on the application of CD4+ T lymphocyte rapid detection method in treating AIDS

Objective: This study aimed to investigate CD4+ T lymphocyte rapid detection method in treating AIDS. Methods: 98 AIDS patients admitted to our hospital from February 2018 to December 2019 were selected as the research group (RG), and 95 healthy patients as the control group (CG). The contents of CD4+ T lymphocytes and total lymphocytes in the peripheral blood of the two groups were detected by flow cytometry, the diagnostic value of CD4+ T lymphocytes in AIDS patients was analyzed by ROC curve, and the contents of IL-10 and TGF-beta 1 in the two groups were detected by ELISA. The changes of CD4+ T lymphocyte content in AIDS patients 1 month, 3 months and 6 months after intervention were detected by flow cytometry. Pearson correlation coefficient was applied to test the correlation of CD4+ T lymphocytes with total lymphocytes, IL-10 and TGF-beta 1, respectively. Multivariate logistic regression analysis was applied to test the risk factors affecting poor prognosis of AIDS patients. Results: CD4+ T lymphocytes and total lymphocytes in the RG were evidently lower than those in the CG, while IL-10 and TGF-beta 1 were higher than those in the CG. AUC of CD4+ T lymphocytes in AIDS diagnosis was 0.806. CD4+ T lymphocyte was positively correlated with the total lymphocytes and negatively correlated with IL-10 and TGF-beta 1. CD4+ T lymphocytes were enhanced, and IL-10 and TGF-beta 1 were reduced in AIDS patients after HAART treatment. Age, clinical stage, CD4+ T lymphocyte count, IL-10 and TGF-beta 1 were independent risk factors for poor prognosis of AIDS patients. Conclusion: CD4+ T lymphocyte detection can be used for early diagnosis of AIDS. It is an indicator of the therapeutic effect of HAART and immune function reconstruction in patients with AIDS and acts on the adverse prognosis of AIDS patients.