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Improved hydrogen production and biomass through the co-cultivation of Chlamydomonas reinhardtii and Bradyrhizobium japonicum
被引:51
作者:
Xu, Lili
[1
,2
,3
]
Li, Dezhi
[3
]
Wang, Quanxi
[1
]
Wu, Shuangxiu
[2
]
机构:
[1] Shanghai Normal Univ, Coll Life & Environm Sci, Dept Biol, Guilin Rd 100, Shanghai 200234, Peoples R China
[2] Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China
[3] E China Normal Univ, Sch Ecol & Environm Sci, Shanghai 200241, Peoples R China
基金:
美国国家科学基金会;
关键词:
Chlamydomonas reinhardtii;
Bradyrhizobium japonicum;
Co-culture;
Hydrogen production;
Biomass;
GREEN-ALGA;
LACTOBACILLUS-AMYLOVORUS;
LIGHT-INTENSITY;
EXPRESSION;
PHOTOPRODUCTION;
FERMENTATION;
BIOHYDROGEN;
CHLOROPLAST;
GENERATION;
BACTERIA;
D O I:
10.1016/j.ijhydene.2016.04.009
中图分类号:
O64 [物理化学(理论化学)、化学物理学];
学科分类号:
070304 ;
081704 ;
摘要:
Three algae strains Chlamydomonas reinhardtii hemHc-lbac (transgenic), cc124 and cc503-were co-cultured with Bradyrhizobium japonicum to improve H-2 production. The maximum H2 productions of the three different co-cultures were 3.5-fold, 17 -foldand 4.4-fold of the pure algal cultures. Meanwhile, the biomass of each of the algae strains was increased by B. japonicum. Furthermore, the respiratory rates of the co-cultures were 1.4-fold, 1.2-fold and 2.6-fold higher than the controls, respectively; the in vitro maximum hydrogenase activities of the co-cultures were 1.1-fold higher, equal to 2.4-fold higher than those of the controls, respectively, and the in vivo maximum hydrogenase activities of the co-cultures were 1.5-fold, 3.8-fold and 2.1-fold higher than the controls, respectively. The maximum starch content of the co-cultures were 8.3-fold, 8.4-fold and 4.4-fold higher than the content of the controls, respectively. Therefore, B. japonicum improved the H2 production of the co-cultures by increasing the biomass, respiratory rates, hydrogen activity and starch content of the co-cultures relative to those of pure algae cultures. (C) 2016 Published by Elseiver Ltd on behalf of Hydrogen Energy Publications LLC.
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页码:9276 / 9283
页数:8
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