Improved hydrogen production and biomass through the co-cultivation of Chlamydomonas reinhardtii and Bradyrhizobium japonicum

被引:51
作者
Xu, Lili [1 ,2 ,3 ]
Li, Dezhi [3 ]
Wang, Quanxi [1 ]
Wu, Shuangxiu [2 ]
机构
[1] Shanghai Normal Univ, Coll Life & Environm Sci, Dept Biol, Guilin Rd 100, Shanghai 200234, Peoples R China
[2] Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China
[3] E China Normal Univ, Sch Ecol & Environm Sci, Shanghai 200241, Peoples R China
基金
美国国家科学基金会;
关键词
Chlamydomonas reinhardtii; Bradyrhizobium japonicum; Co-culture; Hydrogen production; Biomass; GREEN-ALGA; LACTOBACILLUS-AMYLOVORUS; LIGHT-INTENSITY; EXPRESSION; PHOTOPRODUCTION; FERMENTATION; BIOHYDROGEN; CHLOROPLAST; GENERATION; BACTERIA;
D O I
10.1016/j.ijhydene.2016.04.009
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Three algae strains Chlamydomonas reinhardtii hemHc-lbac (transgenic), cc124 and cc503-were co-cultured with Bradyrhizobium japonicum to improve H-2 production. The maximum H2 productions of the three different co-cultures were 3.5-fold, 17 -foldand 4.4-fold of the pure algal cultures. Meanwhile, the biomass of each of the algae strains was increased by B. japonicum. Furthermore, the respiratory rates of the co-cultures were 1.4-fold, 1.2-fold and 2.6-fold higher than the controls, respectively; the in vitro maximum hydrogenase activities of the co-cultures were 1.1-fold higher, equal to 2.4-fold higher than those of the controls, respectively, and the in vivo maximum hydrogenase activities of the co-cultures were 1.5-fold, 3.8-fold and 2.1-fold higher than the controls, respectively. The maximum starch content of the co-cultures were 8.3-fold, 8.4-fold and 4.4-fold higher than the content of the controls, respectively. Therefore, B. japonicum improved the H2 production of the co-cultures by increasing the biomass, respiratory rates, hydrogen activity and starch content of the co-cultures relative to those of pure algae cultures. (C) 2016 Published by Elseiver Ltd on behalf of Hydrogen Energy Publications LLC.
引用
收藏
页码:9276 / 9283
页数:8
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