Neuronal Expression of the Human Neuropeptide S Receptor NPSR1 Identifies NPS-Induced Calcium Signaling Pathways

被引：27
作者：
Erdmann, Frank [1 ]
Kuegler, Sebastian [2 ]
Blaesse, Peter [1 ]
Lange, Maren D. [1 ]
Skryabin, Boris V. [3 ,4 ]
Pape, Hans-Christian [1 ]
Juengling, Kay [1 ]
机构：
[1] Univ Munster, Inst Physiol 1, D-48149 Munster, Germany
[2] Univ Med Gottingen, Dept Neurol, Ctr Mol Physiol Brain, D-37073 Gottingen, Germany
[3] Univ Munster, Inst Expt Pathol, ZMBE, D-48149 Munster, Germany
[4] Univ Munster, Interdisciplinary Clin Res Ctr, D-48149 Munster, Germany
来源：
PLOS ONE | 2015年 / 10卷 / 02期
关键词：
ENDOPLASMIC-RETICULUM; ANXIETY DISORDERS; PANIC DISORDER; CRAC CHANNEL; FOOD-INTAKE; GENE; POLYMORPHISM; ACTIVATION; AMYGDALA; STIM1;
D O I：
10.1371/journal.pone.0117319
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The neuropeptide S (NPS) system was discovered as a novel neurotransmitter system a decade ago and has since been identified as a key player in the modulation of fear and anxiety. Genetic variations of the human NPS receptor (NPSR1) have been associated with pathologies like panic disorders. However, details on the molecular fundamentals of NPSR1 activity in neurons remained elusive. We expressed NPSR1 in primary hippocampal cultures. Using single-cell calcium imaging we found that NPSR1 stimulation induced calcium mobilization from the endoplasmic reticulum via activation of IP3 and ryanodine receptors. Store-operated calcium channels were activated in a downstream process mediating entry of extracellular calcium. We provide the first detailed analysis of NPSR1 activity and the underlying intracellular pathways with respect to calcium mobilization in neurons.
引用
收藏
页数：19
相关论文
共 18 条
  • [1] Neuropeptide S (NPS) variants modify the signaling and risk effects of NPS Receptor 1 (NPSR1) variants in asthma
    Acevedo, Nathalle
    Ezer, Sini
    Merid, Simon Kebede
    Gaertner, Vincent D.
    Soderhall, Cilia
    D'Amato, Mauro
    Kabesch, Michael
    Melen, Erik
    Kere, Juha
    Pulkkinen, Ville
    PLOS ONE, 2017, 12 (05):
  • [2] Abnormal response to stress and impaired NPS-induced hyperlocomotion, anxiolytic effect and corticosterone increase in mice lacking NPSR1
    Zhu, Hongyan
    Mingler, Melissa K.
    McBride, Melissa L.
    Murphy, Andrew J.
    Valenzuela, David M.
    Yancopoulos, George D.
    Williams, Michael T.
    Vorhees, Charles V.
    Rothenberg, Marc E.
    PSYCHONEUROENDOCRINOLOGY, 2010, 35 (08) : 1119 - 1132
  • [3] Neuropeptide S receptor (NPSR1) gene variation modulates response inhibition and error monitoring
    Beste, Christian
    Konrad, Carsten
    Uhlmann, Christina
    Arolt, Volker
    Zwanzger, Peter
    Domschke, Katharina
    NEUROIMAGE, 2013, 71 : 1 - 9
  • [4] Sex-specific association between functional neuropeptide S receptor gene (NPSR1) variants and cortisol and central stress responses
    Streit, Fabian
    Akdeniz, Ceren
    Haddad, Leila
    Kumsta, Robert
    Entringer, Sonja
    Frank, Josef
    Yim, Ilona S.
    Zaenkert, Sandra
    Witt, Stephanie H.
    Kirsch, Peter
    Rietschel, Marcella
    Wuest, Stefan
    PSYCHONEUROENDOCRINOLOGY, 2017, 76 : 49 - 56
  • [5] Lack of Association between Neuropeptide S Receptor 1 Gene (NPSR1) and Eczema in Five European Populations
    Ekelund, Elisabeth
    Bradley, Maria
    Weidinger, Stephan
    Jovanovic, Dragan L.
    Johansson, Catharina
    Lindgren, Cecilia M.
    Todorova, Antonia
    Jakob, Thilo
    Illig, Thomas
    von Mutius, Erika
    Braun-Fahrlaender, Charlotte
    Doekes, Gert
    Riedler, Josef
    Schehynius, Annika
    Pershagen, Goeran
    Kockum, Ingrid
    Kere, Juha
    ACTA DERMATO-VENEREOLOGICA, 2009, 89 (02) : 115 - 121
  • [6] The functional coding variant Asn107Ile of the neuropeptide S receptor gene (NPSR1) influences age at onset of obsessive-compulsive disorder
    Lennertz, Leonhard
    Franke, Petra E.
    Grabe, Hans Joergen
    Rampacher, Friederike
    Schulze-Rauschenbach, Svenja
    Guttenthaler, Vera
    Ruhrmann, Stephan
    Pukrop, Ralf
    Klosterkoetter, Joachim
    Falkai, Peter
    Maier, Wolfgang
    Wagner, Michael
    Moessner, Rainald
    INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY, 2013, 16 (09) : 1951 - 1958
  • [7] The functional coding variant Asn107Ile of the neuropeptide S receptor gene (NPSR1) is associated with schizophrenia and modulates verbal memory and the acoustic startle response
    Lennertz, Leonhard
    Quednow, Boris B.
    Schuhmacher, Anna
    Petrovsky, Nadine
    Frommann, Ingo
    Schulze-Rauschenbach, Svenja
    Landsberg, Martin W.
    Steinbrecher, Anja
    Hoefels, Susanne
    Pukrop, Ralf
    Klosterkoetter, Joachim
    Franke, Petra E.
    Woelwer, Wolfgang
    Gaebel, Wolfgang
    Haefner, Heinz
    Maier, Wolfgang
    Wagner, Michael
    Moessner, Rainald
    INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY, 2012, 15 (09) : 1205 - 1215
  • [8] Signaling pathways involved in the regulation of TNFα-induced toll-like receptor 2 expression in human gingival fibroblasts
    Davanian, Haleh
    Bage, Tove
    Lindberg, Johan
    Lundeberg, Joakim
    Concha, Hernan Q.
    Chen, Margaret Sallberg
    Yucel-Lindberg, Tulay
    CYTOKINE, 2012, 57 (03) : 406 - 416
  • [9] The actin cytoskeleton diffeirentially regulates NG115-401L cell ryanodine receptor and inositol 1,4,5-trisphosphate receptor induced calcium signaling pathways
    Bose, Diptiman D.
    Thomas, David W.
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 379 (02) : 594 - 599
  • [10] Cadmium-induced apoptosis in the BJAB human B cell line: Involvement of PKC/ERK1/2/JNK signaling pathways in HO-1 expression
    Nemmiche, Said
    Chabane-Sari, Daoudi
    Kadri, Malika
    Guiraud, Pascale
    TOXICOLOGY, 2012, 300 (03) : 103 - 111
12