Physiological and immune response in the gills of Litopenaeus vannamei exposed to acute sulfide stress

被引:29
|
作者
Duan, Yafei [1 ]
Wang, Yun [1 ]
Dong, Hongbiao [1 ]
Li, Hua [1 ]
Liu, Qingsong [1 ]
Zhang, Jiasong [1 ]
Xiong, Dalin [1 ]
机构
[1] Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Key Lab Fishery Ecol & Environm, South China Sea Fisheries Res Inst,Minist Agr & R, Guangzhou 510300, Guangdong, Peoples R China
关键词
Crustacean; Environmental pollutants; Osmoregulation; Respiratory metabolic; Immune; SHRIMP FENNEROPENAEUS-CHINENSIS; BLACK TIGER SHRIMP; MUD CRAB; VIBRIO-ALGINOLYTICUS; OXIDATIVE STRESS; HYDROGEN-SULFIDE; PENAEUS-MONODON; TOXICITY; METABOLISM; SUSCEPTIBILITY;
D O I
10.1016/j.fsi.2018.07.018
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Sulfide is a harmful environmental pollutant that affects the survival and immunity of shrimps. The gill is important for shrimp respiratory and osmotic adjustment, the physiological and immune homeostasis of the organ can be influenced by sulfide. In this study, we investigated the acute toxicity of sulfide (5 mg/L) on the morphology, physiological and immune response in the gills of Litopenaeus vannamei. H&E stain showed that sulfide stress damaged the gills histological structure. Specifically, osmoregulation capacity including of Na+/K+-ATPase and Ca2+/Mg2+-ATPase activity was both increased at 6 h and 12 h, and decreased at 72 h; the contents of free amino acid including of Gly, Pro, Ser, Thr and Ala were decreased at 72 h. Respiratory metabolic enzymes, such as cytochrome c oxidase and succcinate dehydrogenase activity was decreased at 12 h-72 h, while fumarate reductase and lactate dehydrogenase activity kept a higher level at 12 h-72 h. Significant variations in the activities of immune enzymes (acid phosphatase, alkaline phosphatase, total antioxidant capacity and lysozyme). The expression of immune-related genes (heat shock protein 70, thioredoxin and caspase-3) was increased at first and then decreased, while hypoxia inducible factor 1 alpha kept a higher level at 6 h-72 h. These results revealed that sulfide stress influenced the L. vannamei gills physiological and immune function by damaging histological structure, and confusing osmoregulation, respiratory metabolic and immune capacity.
引用
收藏
页码:161 / 167
页数:7
相关论文
共 50 条
  • [1] Intestine oxidative stress and immune response to sulfide stress in Pacific white shrimp Litopenaeus vannamei
    Duan, Yafei
    Dong, Hongbiao
    Wang, Yun
    Li, Hua
    Liu, Qingsong
    Zhang, Yue
    Zhang, Jiasong
    FISH & SHELLFISH IMMUNOLOGY, 2017, 63 : 201 - 207
  • [2] Changes in Physiological Homeostasis in the Gills of Litopenaeus vannamei Under Carbonate Alkalinity Stress and Recovery Conditions
    Xiao, Meng
    Nan, Yuxiu
    Yang, Yukai
    Li, Hua
    Duan, Yafei
    FISHES, 2024, 9 (11)
  • [3] Transcriptomic response to low salinity stress in gills of the Pacific white shrimp, Litopenaeus vannamei
    Hu, Dongxu
    Pan, Luqing
    Zhao, Qun
    Ren, Qin
    MARINE GENOMICS, 2015, 24 : 297 - 304
  • [4] Transcriptomic response to low pH stress in gills of the pacific white shrimp, Litopenaeus vannamei
    Li, Hongmei
    Meng, Qilin
    Chen, Ting
    Ren, Chunhua
    Huang, Wen
    Huo, Da
    Jiang, Xiao
    Hu, Chaoqun
    AQUACULTURE RESEARCH, 2020, 51 (01) : 175 - 186
  • [5] Transcriptomic and microbiota response on Litopenaeus vannamei intestine subjected to acute sulfide exposure
    Duan, Yafei
    Wang, Yun
    Liu, Qingsong
    Xiong, Dalin
    Zhang, Jiasong
    FISH & SHELLFISH IMMUNOLOGY, 2019, 88 : 335 - 343
  • [6] Toxicity of Ammonia Stress on the Physiological Homeostasis in the Gills of Litopenaeus vannamei under Seawater and Low-Salinity Conditions
    Nan, Yuxiu
    Xiao, Meng
    Duan, Yafei
    Yang, Yukai
    BIOLOGY-BASEL, 2024, 13 (04):
  • [7] Immune response and oxidative stress of shrimp Litopenaeus vannamei at different moon phases
    Carlos Bautista-Covarrubias, Juan
    Anely Zamora-Ibarra, Patricia
    Apreza-Burgos, Elizabeth
    Nallelhy Rodriguez-Ocampo, Angelica
    Peraza-Gomez, Viridiana
    Armando Lopez-Sanchez, Jose
    Manuel Pacheco-Vega, Juan
    Pablo Gonzalez-Hermoso, Juan
    Gabriel Frias-Espericueta, Martin
    FISH & SHELLFISH IMMUNOLOGY, 2020, 106 : 591 - 595
  • [8] Metabolic and immune responses in Pacific whiteleg shrimp Litopenaeus vannamei exposed to a repeated handling stress
    Mercier, Laurence
    Palaclos, Elena
    Campa-Cordova, Angel I.
    Tovar-Ramirez, Dariel
    Hernandez-Herrera, Roberto
    Racotta, Ilie S.
    AQUACULTURE, 2006, 258 (1-4) : 633 - 640
  • [9] Alternative Splicing Reveals Acute Stress Response of Litopenaeus vannamei at High Alkalinity
    Xiang Shi
    Ruiqi Zhang
    Zhe Liu
    Guiyan Zhao
    Jintao Guo
    Xue Mao
    Baoyi Fan
    Marine Biotechnology, 2024, 26 : 103 - 115
  • [10] Alternative Splicing Reveals Acute Stress Response of Litopenaeus vannamei at High Alkalinity
    Shi, Xiang
    Zhang, Ruiqi
    Liu, Zhe
    Zhao, Guiyan
    Guo, Jintao
    Mao, Xue
    Fan, Baoyi
    MARINE BIOTECHNOLOGY, 2024, 26 (01) : 103 - 115