Optimisation of Bee Pollen Extraction to Maximise Extractable Antioxidant Constituents

被引：30
作者：
Lawag, Ivan Lozada [1 ,2 ]
Yoo, Okhee [2 ]
Lim, Lee Yong [2 ]
Hammer, Katherine [1 ,3 ]
Locher, Cornelia [1 ,2 ]
机构：
[1] Univ Western Australia, Cooperat Res Ctr Honey Bee Prod Ltd CRC HBP, Agr North M085, Perth, WA 6009, Australia
[2] Univ Western Australia, Sch Allied Hlth, Div Pharm, Curnow Bldg M315, Perth, WA 6009, Australia
[3] Univ Western Australia, Sch Biomed Sci, M Block QEII Med Ctr, Monash Ave, Perth, WA 6009, Australia
来源：
ANTIOXIDANTS | 2021年 / 10卷 / 07期
关键词：
bee pollen; Jarrah; Marri; extraction; 2-diphenyl-1-picrylhydrazyl (DPPH); ferric reducing antioxidant power (FRAP); total phenolic content (TPC); optimisation; antioxidant activity; review; multilevel factor analysis (MFA); RADICAL SCAVENGING ACTIVITY; HONEYBEE-COLLECTED POLLEN; PREVENT LIPID OXIDATION; CHEMICAL-COMPOSITION; PHENOLIC-COMPOUNDS; PALYNOLOGICAL ORIGIN; BIOACTIVE COMPOUNDS; VOLATILE COMPOUNDS; DIFFERENT REGIONS; BOTANICAL ORIGIN;
D O I：
10.3390/antiox10071113
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper presents the findings of a comprehensive review on common bee pollen processing methods which can impact extraction efficiency and lead to differences in measured total phenolic content (TPC) and radical scavenging activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) data. This hampers the comparative analysis of bee pollen from different floral sources and geographical locations. Based on the review, an in-depth investigation was carried out to identify the most efficient process to maximise the extraction of components for measurement of TPC, DPPH and FRAP antioxidant activity for two bee pollen samples from western Australia (Jarrah and Marri pollen). Optimisation by Design of Experiment with Multilevel Factorial Analysis (Categorical) modelling was performed. The independent variables included pollen pulverisation, the extraction solvent (70% aqueous ethanol, ethanol, methanol and water) and the extraction process (agitation, maceration, reflux and sonication). The data demonstrate that non-pulverised bee pollen extracted with 70% aqueous ethanol using the agitation extraction method constitute the optimal conditions to maximise the extraction of phenolics and antioxidant principles in these bee pollen samples.
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页数：41
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