Sphingosine-1-phosphate inhibits actin nucleation and pseudopodium formation to control cell motility of mouse melanoma cells

被引:26
作者
Yamamura, S
Sadahira, Y
Ruan, FQ
Hakomori, S
Igarashi, Y
机构
[1] BIOMEMBRANE INST,SEATTLE,WA 98119
[2] UNIV WASHINGTON,DEPT PATHOBIOL,SEATTLE,WA 98195
[3] UNIV WASHINGTON,DEPT MICROBIOL,SEATTLE,WA 98195
关键词
sphingosine-1-phosphate; actin nucleation; pseudopodium; cell motility;
D O I
10.1016/0014-5793(96)00175-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sphingosine-1-phosphate (Sph-1-P), the initial product of sphingosine (Sph) catabolism, has been reported to inhibit motility of mouse melanoma B16/F1 and other types of cells at very low concentrations (10-100 nM). Sph-1-P (100 nM-1 mu M) inhibited pseudopodium formation by blocking polymerization and reorganization of actin filaments in newly formed pseudopodia, and reduced F-actin by similar to 25% in F1 cells. A pyrene-labeled actin nucleation assay revealed that Sph-1-P (100 nM) inhibits actin nucleation mediated by F1 cell plasma membranes. These results suggest that Sph-1-P interacts with molecules associated with actin nucleation to inhibit reorganization of pseudopodium formation and cell motility.
引用
收藏
页码:193 / 197
页数:5
相关论文
共 30 条
[1]   ACTIVATION OF THE HUMAN C-KIT PRODUCT BY LIGAND-INDUCED DIMERIZATION MEDIATES CIRCULAR ACTIN REORGANIZATION AND CHEMOTAXIS [J].
BLUMEJENSEN, P ;
CLAESSONWELSH, L ;
SIEGBAHN, A ;
ZSEBO, KM ;
WESTERMARK, B ;
HELDIN, CH .
EMBO JOURNAL, 1991, 10 (13) :4121-4128
[2]   AN ACTIN-NUCLEATING ACTIVITY IN POLYMORPHONUCLEAR LEUKOCYTES IS MODULATED BY CHEMOTACTIC PEPTIDES [J].
CARSON, M ;
WEBER, A ;
ZIGMOND, SH .
JOURNAL OF CELL BIOLOGY, 1986, 103 (06) :2707-2714
[3]   THE INTEGRAL MEMBRANE-PROTEIN, PONTICULIN, ACTS AS A MONOMER IN NUCLEATING ACTIN ASSEMBLY [J].
CHIA, CP ;
SHARIFF, A ;
SAVAGE, SA ;
LUNA, EJ .
JOURNAL OF CELL BIOLOGY, 1993, 120 (04) :909-922
[4]   RELATIONSHIP OF F-ACTIN DISTRIBUTION TO DEVELOPMENT OF POLAR SHAPE IN HUMAN POLYMORPHONUCLEAR NEUTROPHILS [J].
COATES, TD ;
WATTS, RG ;
HARTMAN, R ;
HOWARD, TH .
JOURNAL OF CELL BIOLOGY, 1992, 117 (04) :765-774
[5]  
DESAI NN, 1992, J BIOL CHEM, V267, P23122
[6]   CHEMOTAXIS IN EUKARYOTIC CELLS - A FOCUS ON LEUKOCYTES AND DICTYOSTELIUM [J].
DEVREOTES, PN ;
ZIGMOND, SH .
ANNUAL REVIEW OF CELL BIOLOGY, 1988, 4 :649-686
[7]   POLYMERIZATION OF ACTIN - MECHANISM OF THE MG-2+-INDUCED PROCESS AT PH-8 AND 20-DEGREES-C [J].
FRIEDEN, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (21) :6513-6517
[8]   THE SYNTHESIS AND CONFIGURATIONAL STABILITY OF DIFFERENTIALLY PROTECTED BETA-HYDROXY-ALPHA-AMINO ALDEHYDES [J].
GARNER, P ;
PARK, JM .
JOURNAL OF ORGANIC CHEMISTRY, 1987, 52 (12) :2361-2364
[9]   INTRACELLULAR CALCIUM RELEASE MEDIATED BY SPHINGOSINE DERIVATIVES GENERATED IN CELLS [J].
GHOSH, TK ;
BIAN, J ;
GILL, DL .
SCIENCE, 1990, 248 (4963) :1653-1656
[10]  
GHOSH TK, 1994, J BIOL CHEM, V269, P22628