Cell swelling-induced signaling for insulin secretion bypasses steps involving g proteins and PLA2 and is N-ethylmaleimide insensitive

Background: This study was undertaken to examine putative mechanisms of calcium independent signal transduction pathway of cell swelling-induced insulin secretion. Methods: The role of phospholipase A 2, G proteins, and soluble N-ethylmaleimide-sensitive-factor attachment protein receptor ( SNARE) in insulin secretion induced by 30% hypotonic medium was studied using isolated rat pancreatic islets. Results: In contrast to glucose stimulation, osmotically induced insulin secretion from pancreatic islets was not inhibited by 10 mu mol/l bromoenol lactone, an iPLA 2 (Ca2+ independent phospholipase) inhibitor. Similarly, preincubation of islets for 20 hours with 25 mu g/ml mycophenolic acid to inhibit GTP synthesis fully abolished glucose-induced insulin secretion but was without effect on hypotonicity stimulated insulin release. Glucose-induced insulin secretion was prevented by preincubation with 20 nmol/l tetanus toxin (TeTx), a metalloprotease inactivating soluble SNARE. Cell swelling-induced insulin secretion was inhibited by TeTx in the presence of calcium ions but not in calcium depleted medium. The presence of N-ethylmaleimide (NEM, 5 mmol/l, another inhibitor of SNARE proteins) in the medium resulted in high basal insulin secretion and lacking response to glucose stimulation. In contrast, high basal insulin secretion from NEM treated islets further increased after hypotonic stimulation. Conclusion: G proteins and iPLA(2) - putative mediators of Ca2+ independent signaling pathway participate in glucose but not in hypotonicity-induced insulin secretion. Hypotonicity-induced insulin secretion is sensitive to clostridial neurotoxin TeTx but is resistant to NEM. Copyright (c) 2007 S. Karger AG, Basel.