Determination of free and conjugated forms of endocrine-disrupting chemicals in human biological fluids by GC-MS

被引：30

作者：

Azzouz, Abdelmonaim ^{[1
]}

Rascon, Andres J. ^{[1
]}

Ballesteros, Evaristo ^{[1
]}

机构：

[1] Univ Jaen, EPS Linares, Dept Phys & Analyt Chem, E-23700 Linares, Jaen, Spain

来源：

BIOANALYSIS | 2016年 / 8卷 / 11期

关键词：

derivatization; endocrine-disrupting chemicals; enzymatic hydrolysis; GC-MS; long-term dietary exposure; matrix effect; SPE; TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; LIQUID-LIQUID MICROEXTRACTION; MEASURING ENVIRONMENTAL PHENOLS; BISPHENOL-A CONCENTRATIONS; P-HYDROXYBENZOIC ACID; HPLC-MS/MS METHOD; GAS-CHROMATOGRAPHY; HUMAN URINE; HUMAN SERUM;

D O I：

10.4155/bio-2015-0008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Humans are exposed to hazardous substances including endocrine-disrupting chemicals (EDCs). These compounds have been associated with some diseases such as cancer and ascribed adverse effects on life-essential organs. Results: The method, which allows the determination of both free and conjugated forms of EDCs, involves the liquid-liquid extraction from the sample with ethyl acetate, followed by its preconcentration and clean-up by SPE in a continuous system for the subsequent determination by GC-MS. The proposed method affords very low LODs and RSD. Conclusion: This allowed its successful application to the determination of EDCs in human urine, blood and breast milk. The most frequently founded were methylparaben, ethylparaben, bisphenol A and triclosan.

引用

页码：1145 / 1158

页数：14

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